Abstract：Acquired immune deficiency syndrome (AIDS) is increasing its negative influences on human society and economy.In the present paper, HIV-1 cell fusion peptide inhibitor C22 was expressed and purified based on the C-terminal sequence of HIV-l membrane fusion glycoprotein gp41.The gene coding for C22 was totally synthesized using gp41 gene as a template and amplified by PCR.The cloned C22 gene was confirmed by restriction endonuclease and sequence analysis and then cloned into plasmid pTMHa30-51.The prepared plasmid was transformed into E.coli BL21 (DE3) and the expressing products were analyzed on SDS-PAGE and tested with mass spectrum.The results indicated that C22 showed high HIV-1 fusion inhibiting capacity, meanwhile, with good thermal stability and water-solubility, and showed no cell toxicity in tested concentrations.The spectral characteristics were tested with circular dichroism (CD).When treated at different temperature in solution condition, the content of α helix of C22 increased at 37 ℃ while decreased sharply at 80 ℃.The peak value changed significantly with different pH values.The content of α helix of C22 decreased as pH varied toward acid and alkali and the random coiling increased, which led to a relaxed structure.This result indicated that the C22 structure is stable with pH 6.This research may provide a theoretic foundation for the new type HIV-1 peptide inhibitor designing and spectral characteristics study.
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