Study on Compatibility of Coptidis-Evodia Couples by FTIR Spectroscopy
WU Yan-wen1, SUN Su-qin2, XIAO Xiao-he3, ZHANG Jing-hua1, JIN Cheng3
1. Beijing Center for Physical and Chemical Analysis, Beijing100089, China 2. Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing100084, China 3. PLA Institute of Chinese Materia Medica, 302 Hospital of PLA, Beijing100039, China
Abstract:Combining unique macro-fingerprint characteristics of infrared spectroscopy (IR) with the overall concept of traditional Chinese medicine (TCM) theory, the authors seek to establish a new, rapid and economic method for research on Chinese herb preparation (CHP), which could reflect the whole and integration concept of TCM theory and also be widely applied in TCM research. In the present paper, four coptidis-evodia couples, e.g. Zuo-jin-wan (coptidis∶evodia=6∶1), Gan-lu-san (coptidis∶evodia=2∶1), Zhu-yu-wan (coptidis∶evodia=1∶1) and Fan-zuo-jin (coptidis∶evodia=1∶6), were extracted by boiling water and 80% alcohol, and the FTIR spectra of those CHPs were compared and analyzed in detail. The characteristic absorbance bands of coptidis and evodia were assigned in the FTIR spectra of the four coptidis-evodia couples. The results showed that rhizoma coptidis owns absolute superiority in both extracts of Zuo-jin-wan and Gan-lu-san since the proportions of rhizoma coptidis in the two couples are higher than those of fructus evodiae. With regard to Zhu-yu-wan, the proportions of two ingredients are the same. However, the contribution rate of coptidis is higher than that of evodiae in water extract of Zhu-yu-wan. In addition, coptidis also shows higher contribution rate than that of evodia in alcohol extract of Fan-zuo-jin. The spectroscopic technique described here is a simple and rapid analytical technique, which could provide valuable chemical information about the whole coptidis-evodia CHPs.
Key words:Chinese herb preparation;Compatibility;FTIR;Coptidis-evodia
武彦文1,孙素琴2,肖小河3,张经华1,金城3 . 黄连-吴茱萸药对不同配伍方式的红外光谱研究[J]. 光谱学与光谱分析, 2009, 29(07): 1797-1800.
WU Yan-wen1, SUN Su-qin2, XIAO Xiao-he3, ZHANG Jing-hua1, JIN Cheng3 . Study on Compatibility of Coptidis-Evodia Couples by FTIR Spectroscopy . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(07): 1797-1800.
[1] XIAO Pei-gen, XIAO Xiao-he(肖培根,肖小河). China Journal of Chinese Materia Medica(中国中药杂志), 2000, 25(2): 67. [2] SUN Su-qin, ZHOU Qun, QIN Zhu(孙素琴, 周群, 秦竹). Atlas of Two-Dimensional Correlation Infrared Spectroscopy for Traditional Chinese Medicine Identification(中药二维相关红外光谱鉴定图集). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2003. [3] Zhou Qun, Sun Suqin, Zuo Lin. Vibrational. Spectrosc. 2004, 36: 207. [4] YU Lu, SUN Su-qin, FAN Ke-feng, et al. Spetrochim. Acta A: Mol. Biomol. Spectrosc. 2005, 62(1-3): 22. [5] Liu Hongxia, Sun Suqin, Lü Guanghua, et al. Vibrational. Spectrosc., 2006, 40: 202. [6] Yap K Y L, Chan S Y, Lim C S, Food Research International, 2007, 40: 643. [7] Wu Yanwen, Sun Suqin, Zhao Jing, et al. J. Mol. Struct., 2008, 883:48. [8] Wu Yanwen, Sun Suqin, Zhou Qun, et al. J. Pharmaceutieal Biomedieal Analysis, 2008, 46: 98. [9] Wu Yanwen, Sun Suqin, Zhou Qun, et al. J. Mol. Struct., 2008, 882: 107. [10] Pharmacopoeia Committee of the Ministry of Public Health of the People’s Republic of China(中华人民共和国卫生部药典委员会). Pharmacopoeia of the Ministry of Public Health of the People’s Republic of ChinaⅠ(中华人民共和国卫生部药典, 第1部). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2005. 213. [11] XIU Yan-feng,XU De-sheng, FENG Yi, et al(修彦凤,徐德生,冯怡,等). Chinese Traditional and Herbal Drugs(中草药),2003, 34: 320. [12] Chu Chanyeo, Sheu S J. J. Chromatogr. A, 1996, 756: 137. [13] Chuang Wuchang, Chu Chanyeo, Sheu S J. J. Chromatogr. A, 1996, 727: 317. [14] Lee Mingchung, Chuang Wuchang, Sheu S J. J. Chromatogr. A, 1996, 755: 113. [15] WU Yan-wen, XIAO Xiao-he, SUN Su-qin, et al(武彦文,肖小河,孙素琴,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2009, 29(1): 93.