Laser Tweezers Raman Spectroscopy Analysis of Liver Cancer Tissue
WANG Yan-jun1, YAO Hui-lu2, WANG Gui-wen2, WANG Yun1, FENG Mei-fu1*
1. State Key Lab of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China 2. Laboratory for Biophysics, Guangxi Academy of Sciences, Nanning 530003, China
Abstract：Single cell laser tweezers Raman spectroscopy (LTRS) has been applied to biology field. In the present article, the authors measured the spectra of liver cancer cells, para-cancer cells and normal hepatocytes using single cell laser tweezer Raman spectroscopy (LTRS) system and compared their average spectra changes. The results showed that the laser tweezers Raman spectroscopy could differentiate specimens of different pathological changes from liver tissue studied. The 1 070 and 1 266 cm-1 peaks obtained from normal hepatocytes were more visible than the same two peaks obtained from liver cancer and para-cancer specimen. The 1 445 cm-1 peak of normal hepatocytes was higher than that of liver cancer cells and para-cancer cells. It is known that the 1 070 cm-1 peak represents lipids and nucleic acids, while 1 266 and 1 445 cm-1 peaks represent lipids and proteins. So, these peak changes may directly reflect the changed biomaterials related to liver carcinogenesis. Thus, single cell laser tweezer Raman spectroscopy may be a nondestructive, rapid and good method to measure and analyze different pathological specimens from liver cancer.
Key words：Liver cancer cells;Normal hepatocytes;Laser Tweezers Raman spectroscopy
王雁军1,姚辉璐2,王桂文2,汪蕴1,丰美福1* . 人肝癌组织细胞的激光光镊拉曼光谱研究[J]. 光谱学与光谱分析, 2009, 29(07): 1881-1883.
WANG Yan-jun1, YAO Hui-lu2, WANG Gui-wen2, WANG Yun1, FENG Mei-fu1* . Laser Tweezers Raman Spectroscopy Analysis of Liver Cancer Tissue. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(07): 1881-1883.
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