Abstract：Raman spectrum was used to study the structure and content of protein，nucleic acid and fat, while EC9706 cells irradiated by 60Co γ-ray were cultivated for 24 h. The results showed that for spectrum intensity and frequency deviation, there were big differences between each exposure group and control group. For the 1 244 cm-1 peak of amide Ⅲ, β folder changed to disordered conformations in the middle dose (4, 5Gy) groups. The 1 341 cm-1 peak of ν (the indole ring of Trp) was red-shifted in every dose group. There was a 2-3 cm-1 red shift at the 782 cm-1 peak in the big dose groups (7, 8Gy). It was showed that the non-hydrogenation of νs (PO-2) was strengthened due to big dose γ-rays radiation. There was a 4 cm-1 blue shift at the 1 446 cm-1 peak of δ (CH2, CH3). It maybe resulted from 60Co γ-rays’ damage to the film of EC9706 cells. The preferable dose of 60Co γ-rays may be found by analyzing the variety of the above-mentioned peaks in some dose groups.
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