Study on Interaction between Heme-Iron of Myoglobin and Metal Ions by Visible Spectroscopy(Ⅰ)
TANG Qian1, 2,ZHENG Xue-fang1*, WANG Jing-yun3, LIU Yuan-yuan1, 2, YUAN Yu-lian2
1. Liaoning Key Lab of Bioorganic Chemistry, Dalian University, Dalian 116622, China 2. College of Bioengineering, Dalian University, Dalian 116622, China 3. School of Environmental & Biological Science, Dalian University of Technology, Dalian 116026, China
Abstract：Myoglobin (Mb) made up of a multipeptides train and a heme prosthetic group is a kind of protein taking charge of O2 stock and distribution in mammal cells, especially in muscle cells. The heme-iron plays a key role in O2 transfer and transport. In the present paper, the direct interaction between heme-iron of myoglobin and additional metal ions [Cu(Ⅱ), Zn(Ⅱ) and Co(Ⅱ)] was studied by UV-Vis spectra. It was found that heme-iron of myoglobin directly interacted with additional Cu(Ⅱ), Zn(Ⅱ) and Co(Ⅱ), these metal ions could drag iron ion out from heme prosthetic group of myoglobin, and subsequently myoglobin became myoglobin derivatives lacking iron ion. At the same time, the effect of the additional metal ions concentration on the direction interaction was studied. It was shown that the direct interaction increased gradually with the amount of external metal ions added. When the ratio of Mb and metal ions is 1∶10, the interaction intension between the three metal ions and Mb is Co(Ⅱ), Zn(Ⅱ) and Cu(Ⅱ) in turn. For the first time, the authors confirmed that the direct interaction has occurred between heme-iron of myoglobin and additional metal ions, and saw about how the metal ions intension affects the direct interaction.
唐乾1, 2,郑学仿1*,王静云3,刘媛媛1, 2,袁玉莲2. 可见光谱法研究肌红蛋白血红素铁与金属离子相互作用(Ⅰ)[J]. 光谱学与光谱分析, 2009, 29(07): 1958-1961.
TANG Qian1, 2,ZHENG Xue-fang1*, WANG Jing-yun3, LIU Yuan-yuan1, 2, YUAN Yu-lian2. Study on Interaction between Heme-Iron of Myoglobin and Metal Ions by Visible Spectroscopy(Ⅰ). SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(07): 1958-1961.
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