Spectroscopic Studies on the Binding of Prulifloxacin and ct-DNA
CHEN Chang-yun1，3, MA Mei-hua1, ZHOU Zhi-hua2, LU Kang1, XIANG Bing-ren3
1. Department of Chemistry, Nanjing Xiaozhuang College, Nanjing 210017, China 2. Department of Chemistry, Nanjing Normal University, Nanjing 210097, China 3. Analysis Center, China Pharmaceutical University， Nanjing 210009, China
Abstract：The interaction mechanism of prulifloxacin(PL) and calf thymus DNA(ct-DNA) was studied by UV spectra, fluorescence spectra, and hydrodynamic measurements. The binding of ct-DNA and different concentrations of PL was discussed with UV, FL, phosphate effect and ion strength. The denaturation temperature and viscosity were measured. It is obvious that there is a hypochromic effect on the UV spectra with the fluorescence intensity decreasing regularly after addition of DNA and a red shift of the maximum emission peak with a static quenching constant of 3.1×104 L·mol-1. The above results show that a binary complex forms between PL and DNA. It is indicated that there is no static-electro interaction between them by phosphate effect. The outer groove binding is suggested by denaturation temperature of DNA increasing (no more than 7 ℃) and viscosity slightly decreasing.
陈昌云1，3,马美华1,周志华2,陆康1,相秉仁3 . 光谱法研究普利沙星与小牛胸腺DNA的结合作用[J]. 光谱学与光谱分析, 2006, 26(10): 1891-1894.
CHEN Chang-yun1，3, MA Mei-hua1, ZHOU Zhi-hua2, LU Kang1, XIANG Bing-ren3 . Spectroscopic Studies on the Binding of Prulifloxacin and ct-DNA. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(10): 1891-1894.
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