Application of L-Cysteine-Capped Nano-ZnS as a Fluorescence Probe to the Determination of Proteins in Human Serum
ZHAO Dan-hua1, LI Yong-xin2,ZHU Chang-qing2
1. Chemistry Department, Huaibei Coal Normal College, Huaibei 235000, China 2. College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, China
Abstract：Nanometer-sized L-cysteine-capped ZnS particles as a fluorescence probe were synthesized, and the effect of proteins on the fluorescence intensity has been investigated. With Δλ=190 nm, the maximum and constant synchronous fluorescence enhancement was produced at 268.0 nm and pH 5.12 in the presence of proteins. A highly sensitive synchronous fluorescence method for the rapid determination of proteins was developed. Under optimum conditions, the calibration curve was linear over the range 0.05-6.0 μg·mL-1 for human serum albumin (HSA), and 0.05-8.0 μg·mL-1 for γ-globulin(γ-G), respectively. The relative standard deviations of seven measurements were 1.90% for 1.0 μg·mL-1 HSA, and 1.65% for 1.0 μg mL-1γ-G.
赵丹华1,李永新2,朱昌青2 . L-半胱氨酸包覆纳米ZnS荧光探针在测定人血清中蛋白质的应用[J]. 光谱学与光谱分析, 2006, 26(07): 1318-1321.
ZHAO Dan-hua1, LI Yong-xin2,ZHU Chang-qing2. Application of L-Cysteine-Capped Nano-ZnS as a Fluorescence Probe to the Determination of Proteins in Human Serum. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(07): 1318-1321.
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