Abstract:In the present paper,the interaction of Magdala red and bovine serum albumin(BSA) was studied by UV-absorption and fluorescence spectrometry. The results of UV-absorption spectrometry show that the way of the interaction of Magdala red and BSA should obey the Scatchard Model. In other words, Magdala should be combined to some certain regions of BSA. Meanwhile, taking advantage of fluorescence quenching, the reaction mechanism of Magdala with BSA was explored. Protein molecule conformation change is discussed by synchronous fluorescence spectrometry.
Key words:Magdala red;Bovine serum albumin;Interaction;Spectrometry study
石燕,叶反帝,郑为完. 麦塔喇红与蛋白质相互作用的光谱研究[J]. 光谱学与光谱分析, 2006, 26(07): 1314-1317.
SHI Yan, YE Fan-di, ZHENG Wei-wan. Spectrometry Study on the Interaction of Magdala Red and Bovine Serum Albumin . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(07): 1314-1317.