1.China Three Gorges University Alan G MacDiarmid Institute for Renewable Energy, Yichang 443002, China 2.College of Mechanical and Material Engineering, China Three Gorges University, Yichang 443002, China
Abstract:The reaction conditions for the determination of bovine serum albumin (BSA) using o-hydroxyphenylfluorone (o-HPF) as fluorescence probe reagent were studied.In the B-R medium at pH 7.90, o-HPF could react with BSA, producing a stable complex compound which resulted in fluorescent quenching of this binary system.The quantitative assay was carried out based on the relation between the degree of fluorescent quenching and the additional amount of BSA.The method has advantages of good selectivity, stability and simplicity.The linear range of o-HPF fluorescence spectrophotometric method was 1.32-18.54 μg· mL-1.Moreover, the mechanism of the reaction BSA and o-HPF was discussed, and the quenching constant, and the thermodynamics constant of the fluorescence effects were calculated.The results showed that the nonconvalent binding forces were the binding force between o-HPF and BSA, and the quenching of BSA to o-HPF was probably a single static quenching process.
Key words:o-hydroxyphenylfluorone (o-HPF);Bovine serum albumin (BSA);Fluorescence spectrophotometry
黄应平1,顾 彦2,方艳芬1,刘玉良1,朱圣姬1,罗光富1. 邻羟基苯基荧光酮荧光法测定牛血清白蛋白[J]. 光谱学与光谱分析, 2008, 28(08): 1887-1890.
HUANG Ying-ping1,GU Yan2,FANG Yan-fen1,LIU Yu-liang1,ZHU Sheng-ji1,LOU Guang-fu1. Study on Determination of Bovine Serum Albumin Using o-Hydroxyphenylfluorone Reagent with Fluorescence Spectrophotometry. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(08): 1887-1890.