Study on the Interaction of Sm(Ⅲ) Complexes of Rutin with Serum Albumin
WU Jin-xiu1, LI Mei1, SONG Yu-min2, LIU Zhao-gang1, HU Yan-hong1
1. College of Rare Earth, Inner Mongolia University of Science and Technology, Baotou 014010, China 2. College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China
Abstract：The binding reaction of rutin-Sm with serum albumin (SA) was investigated by the fluorescence method in physiological condition. The authors studied mainly the quenching mechanism of the fluorescence of SA by rutin-Sm, and calculation of the binding constants KLB of human serum albumin (HSA) and bovine serum albumin(BSA)with rutin-Sm by Lineweaver-Burk equation at different temperatures respectively, then obtained the thermodynamic parameters of HSA and BSA with rutin-Sm according to the calculated binding constants KLB at different temperature, meanwhile the type of binding forces of HSA and BSA with rutin-Sm was determined. The results showed that the emission spectra of BSA(HSA)in the presence and absence of rutin-Sm are different. The emission spectra of BSA(HSA)in the presence of rutin-Sm can be quenched. The quenching mechanism of rutin-Sm to SA was static quenching with non-radiation energy transfer for new complex of SA and rutin-Sm. The binding constants KLB (L·moL-1)were 6.540×105 and 3.265×105 for BSA, and 6.830×105 and 4.665×105 for HSA at 295 K and 310 K respectively. And the type of bonding forces was estimated by the calculation of thermodynamic parameters of the reaction of rutin-Sm with SA at different temperatures, and the result showed that the binding forces were mainly H-bond and Van der Waals between BSA and rutin-Sm due to the ΔH＜0 and ΔS＜0, and the main electrostatic interaction of rutin-Sm and HSA because of ΔH＜0 and ΔS＞0. The effect of rutin-Sm on the conformation of serum albumin was also studied by using synchronous fluorescence spectroscopy. Results indicated that rutin-Sm could be deposited and transported by serum protein in vivo.
Key words：Rutin Sm complexes;Human serum albumin;Bovine serum albumin;Fluorescence spectrum;Thermodynamic parameters
吴锦绣1,李 梅1*,宋玉民2,柳召刚1,胡艳宏1 . 芦丁钐配合物与血清白蛋白的相互作用[J]. 光谱学与光谱分析, 2009, 29(08): 2199-2203.
WU Jin-xiu1, LI Mei1, SONG Yu-min2, LIU Zhao-gang1, HU Yan-hong1 . Study on the Interaction of Sm(Ⅲ) Complexes of Rutin with Serum Albumin. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(08): 2199-2203.
