Study on Structures of Blattela Germanica Allergen, Bla g 2 Expressed by Eukaryotic and Prokaryotic Vectors Using Fluorescence and CD Spectra
LIU Zhi-gang1, ZHU Jian-qi1, HUANG Hai-zhen2, XU Hong1
1. College of Life Science, Shenzhen University, Shenzhen 518060, China 2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
摘要： 在基因工程技术中，采用原核表达系统获得的重组蛋白是以包涵体的形式存在，在细胞内聚集成无生物活性的不溶性颗粒，而采用真核表达系统收获的蛋白已经是经过正确的体内折叠的活性蛋白。文章分别用真核与原核表达系统表达重组变应原，通过对不同来源目的蛋白的荧光光谱和CD谱的比较和分析，对真核系统与原核系统表达出的Bla g 2蛋白的高级构象进行研究，阐明了Bla g 2在溶液中的二级结构，并推断出其三级结构组成类型。
关键词：德国小蠊;变应原;Bla g 2;真核表达;原核表达;荧光光谱;CD谱
Abstract：The recombinant allergen, Bla g 2, was expressed by prokaryotic vector E.coli and eukaryotic vector P.Pastoris. The different structures and configurations of the Bla g 2 from E.coli and P.Pastoris were studied by fluorescence and circular dichroism. The secondary structures of Bla g 2 in solution, and the composition besides the type of its tertiary structure were proposed. These studies help understand the differences between prokaryotic and eukaryotic expression systems, reveal the relationship between the structure and the function of Bla g 2, and improve the production of this significant allergen.
Key words：Blattella germanica;Allergen;Bla g 2;Eukaryotic vector;Prokaryotic vector;Fluorescence spectra;CD spectra
刘志刚1,朱健琦1,黄海珍2,徐 宏1 . 真核表达与原核表达的德国小蠊变应原Bla g 2蛋白结构的光谱学研究[J]. 光谱学与光谱分析, 2006, 26(05): 879-883.
LIU Zhi-gang1, ZHU Jian-qi1, HUANG Hai-zhen2, XU Hong1 . Study on Structures of Blattela Germanica Allergen, Bla g 2 Expressed by Eukaryotic and Prokaryotic Vectors Using Fluorescence and CD Spectra. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(05): 879-883.
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