Spectroscopy Study of the Immobilized Cellulose of Magnetic Nanoparticles Fe3O4
WANG Mei1,SONG Fang1,WANG Shi-long2*,WU Qing-sheng1
1. Department of Chemistry, Tongji University, Shanghai 200092, China 2. School of Life Science and Technology, Tongji University, Shanghai 200092, China
Abstract：Fe3O4 magnetic nanoparticles were synthesized by chemical coprecipitation method using ammonia as the precipitator in the present paper. And then cellulase was immobilized on Fe3O4 magnetic nanoparticles via carbodiimide activation, which was testified by FTIR and lots of repeating catalysis experiments. The morphology of nanoparticles immobilized by cellulase was characterized by TEM, and the activity of cellulase was measured by DNS spectrophotometry. The optimum temperature(60 ℃) and pH value (3.94-5.50) for the catalysis ablility of immobilized cellulose were studied. The result showed that compared with the native enzyme the cellulase immobilized on Fe3O4 magnetic nanoparticles has the advantages of thermal stability, storage stability, and more extensive optimum pH value.
王 玫1,宋 芳1,汪世龙２*,吴庆生1 . 磁性纳米颗粒Fe3O4固定化纤维素酶的光谱学研究[J]. 光谱学与光谱分析, 2006, 26(05): 895-898.
WANG Mei1,SONG Fang1,WANG Shi-long2*,WU Qing-sheng1 . Spectroscopy Study of the Immobilized Cellulose of Magnetic Nanoparticles Fe3O4. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(05): 895-898.
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