Determination of Fifty-Five Elements in Lung Carcinomatous Tissues and Their Pericarcinomatous Tissues by Atomic Spectrometry
XIONG Yi-jie1, OUYANG Li1, 2, LIU Ya-qiong1, 2, XIE Qing1, 2, LIU Hu-sheng1, WANG Jing-yu1, 2*
1. School of Public Health, Peking University, Beijing 100083, China 2. Laboratory of Biometallomics, Center of Medical and Health Analysis, Peking University, Beijing 100083
Abstract：A method for determining 55 elements in human lung tissue was developed. Mixed acid (HNO3∶HClO4) was added into samples, which were digested at room temperature over night, then heated at 180 ℃. Arsenic and selenium in lung tissue were determined by hydride generation atomic fluorescence spectrometry (HG-AFS), potassium, sodium, calcium and magnesium were determined by atomic absorption spectrometer (AAS), while the rest of forty-eight elements were determined by inductively coupled plasma mass spectrometry (ICP-MS), respectively. Reference materials of GBW(E)080193 bovine hepar and GBW09101 human hair were analyzed by the described method. The measured element values in two reference materials accorded with their reference values. The recovery rates for most of the studied elements were 90%-110％. The precisions of the method were 1.7％-10.0％. The concentrations of seventeen elements in the carcinomatous tissues were remarkably different from those in the pericarcinomatous tissues. The method is rapid, simple and accurate.
熊依杰1,欧阳荔1，2,刘雅琼1，2,解清1，2,刘虎生1,王京宇1，2* . 肺癌和癌旁组织中55个元素的原子光谱分析[J]. 光谱学与光谱分析, 2006, 26(11): 2120-2124.
XIONG Yi-jie1, OUYANG Li1, 2, LIU Ya-qiong1, 2, XIE Qing1, 2, LIU Hu-sheng1, WANG Jing-yu1, 2* . Determination of Fifty-Five Elements in Lung Carcinomatous Tissues and Their Pericarcinomatous Tissues by Atomic Spectrometry . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(11): 2120-2124.
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