FTIR Spectrum and Coagulation Enhancement of Exopolysaccharide Secreted by an Antarctica Bacterium Pseudoalteromona sp. Bsi20310
ZHOU Wei-zhi1,2, SHEN Bo-ling1, LIU Sheng-bo2, CHEN Bo3, ZHANG Yu-zhong2
1. School of Environmental Science and Engineering, Shandong University, Jin’an 250100, China 2. State Key Lab of Microbial Technology, Shandong University, Ji’nan 250100, China 3. State Oceanic Adminstration People’s Republic of China Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China
Abstract:Bsi20310 exopolysaccharide (Bsi20310 EPS) was secreted by a bacteria named Pseudoalteromonas sp. Bsi20310, isolated from Antarctic Sea ice. Crude Bsi20310 EPS was prepared by precipitation of the culture solution with ethanol, with proteins removed by using chloroform and butanol preparatorily. The results showed that Bsi20310 EPS improved the FeCl3 coagulation performance on synthetic water-soluble dye reactive red X-3B dyeing wastewater, obviously. The optimum coagulation enhancement of Bsi20310 EPS expressed by decolorization rate is from 16% to 84%, at pH near 10, Fe(Ⅲ) concentration of 0.98 mmol·L-1 and Bsi20310 EPS concentration of 150 mg·L-1, respectively. Fourier transform infrared spectroscopy (FTIR) was used to investigate the functional groups of Bsi20310 EPS, Fe(Ⅲ)-Bsi20310 EPS floc and Fe(Ⅲ)-Bsi20310 EPS-reactive red X-3B floc. The spectra showed that Bsi20310 EPS contained a large number of functional groups such as —OH, —COOH and glycosidic bond. Some certain functional groups of Bsi20310 EPS changed being combined with Fe(Ⅲ) hydrolysate. For instance, narrow peaks at 3 429 and 1 650 cm-1 became wide; the peak at 2 921 cm-1 weakened or disappeared; the peak at 1 242 cm-1 red-shifted slightly; peaks in the region of 1 151-1 038 cm-1 became single and sharp, etc. The change in spectra indicated that —OH, —OOH and glycosidic bond might be the main functional groups. The study suggested a bright prospect of Bsi20310 EPS performing as an approach to safe and effective microbial coagulation enhancement.
[1] FANG Ming-hui, ZHAN Shu-lin, LIN Jun-xiong, et al(方明晖,詹树林,林俊雄,等). Industrial Water Treatment(工业水处理), 2007, 27(2): 26. [2] Kim T H, Park C, Yang J, et al. Journal of Hazardous Materials, 2004, 112(1-2): 95. [3] Aboulhassan M A, Souabi S, Yaacoubi A, et al. Journal of Hazardous Materials, 2006, 138(1,2): 40. [4] Renault F, Sancey B, Badot P M, et al. European Polymer Journal, 2009, 45(5): 1337. [5] LIU Zhong-wei, XIONG Rong-chun, WEI Gang(刘中卫,熊蓉春,魏 刚). Journal of Beijing University of Chemical Technology(北京化工大学学报), 2008, 35(6): 45. [6] Sanghi R, Bhattacharya B, Singh V. Reactive and Functional Polymers, 2007, 67 (6): 495. [7] LI Jiang, SONG Guo-qiang, CHEN Kao-shan, et al(李 江,宋国强,陈靠山,等). Chemical Journal of Chinese Universities(高等学校化学学报), 2008, 29(6): 1149. [8] Ozkan A, Yekeler M. Chemical Engineering and Processing, 2004, 43(7): 873. [9] Li W W, Zhou W Z, Zhang Y Z, et al. Bioresource Technology, 2008, 99(15): 6893. [10] XU Hui-sheng, ZHANG Tie-jun, ZHAO Guang-rong, et al(许会生,张铁军,赵广荣,等). Science and Technology of Food Industry(食品工业科技), 2007, 28(7): 197. [11] LUO Ya-jun, XIAO Xin-feng, WANG Zhao-li(罗娅君,肖新峰,王照丽). Chemistry and Industry of Forest Products(林产化学与工业), 2009, 29(1): 68. [12] Lim J M, Joo J H, Kim H O, et al. Carbohydrate Polymers, 2005, 61(3): 296. [13] Lin S, Rayson G D. Environmental Science and Technology, 1998, 32(10): 1488. [14] GU Xiao-mei, WU Hou-ming, MA Gui-rong(顾笑梅,吴厚铭,马桂荣). Chemical Journal of Chinese Universities(高等学校化学学报), 2004, 25(7): 1288. [15] Ai L, Zhang H, Guo B, et al. Carbohydrate Polymers, 2008, 74(3): 353. [16] Pradhan S, Singh S, Rai L C. Bioresource Technology, 2007, 98(3): 595.