Simultaneous Determination of Glucose, Fructose and Sucrose in Aqueous Solution by Short-Wavelength Near Infrared Spectroscopy
RAO Zhen-hong1,LI Min-zan2,JI Hai-yan2*
1. College of Science, China Agricultural University, Beijing 100094, China 2. College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China
Abstract：The calibration model for simultaneous determination of glucose, fructose and sucrose in aqueous solution was built by partial least squares and short-wavelength near infrared spectroscopy(800-1 100 nm). Twenty five samples in calibration set and 9 samples in prediction set were designed by orthogonal design. Building models from calibration set and validation for prediction set obtained the better results. For concentrations of glucose, fructose and sucrose in the ranges of 12.23-61.14 mg·mL-1,12.50-62.50 mg·mL-1 and 12.09-60.44 mg·mL-1,the relative standard deviations in the calibration set are 1.43%, 4.51% and 1.59% respectively, and the relative standard deviations in the prediction set are 3.40%, 3.73% and 2.80% respectively. The advantages of the method are simple and effective, with low cost for the simultaneous determination of multi-component system. It may be applied in practice easily.
Key words：Short-wavelength near infrared spectroscopy;Partial least square method;Glucose;Fructose;Sucrose
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