Determination of Trace Elements in Cerebrospinal Fluid(CSF) of Patients Suffering Cerebrovascular Disease by Atomic Absorption Spectrometry
SUN Rui-xia1,SU Yong-xiang2,SUN Jian-hui1*
1. Chemistry and Environmental Sciences College of Henan Normal University, Henan Key Laboratory for Environmental Pollution Control, Xinxiang 453007, China 2. Department of Biochemistry and Environmental Engineering, Jiaozuo University, Jiaozuo 454003, China
Abstract：The contents of some trace elements such as zinc, copper, iron and cadmium in cerebrospinal fluid(CSF) of normal persons and the patients who suffered cerebral hemorrhage or infarction were determined directly by atomic absorption spectrometry. The method is simple and convenient with a recovery ratio by standard addition being 97.6% to 104.8%, and a relative standard deviation(RSD) is lower than 5%. The test showed that except for the content copper lower than normal, the patients suffering cerebrovascular disease have much higher contents of zinc, iron and cadmium. The result provides useful data for studying the relation between the contents of these trace elements and cerebrovascular disease, as well as diagnosing, treating and preventing this disease.
Key words：Atomic absorption spectrometry;Cerebrovascular disease;Cerebrospinal fluid;Trace elements
[1] KONG Xiang-rui(孔祥瑞). Nutrition, Physiology and Clinical Meaning of the Essential Trace Element(必需微量元素的营养、生理及临床意义). Hefei: Anhui Science Press(合肥：安徽科学出版社), 1982. 290. [2] LIU Xin-min(刘新民). Practical Endocrinology(实用内分泌学). 北京: 人民军医出版社, 1986. 509. [3] ZHENG Chun-xia, WANG Wen-quan, HU Zhi-lin(郑春霞, 王文全, 胡志林). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2000, 20(2): 368. [4] JU Hong-fang(居红芳). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2002, 22(4): 681. [5] WANG Zai-juan, JIN Feng(汪再娟, 金 锋). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2001, 21(3): 409. [6] SUN Rui-xia, ZHOU Ling-mei, XUE Wang-gang(孙瑞霞, 周玲妹, 薛万刚). Spectroscopy and Spectral Analysia(光谱学与光谱分析), 2002，22(5): 853. [7] HUANG Shun-chao, et al(黄舜超, 等). Guangzhou Medicine(广州医药), 1995, (1): 20. [8] Allen K G D, Klevay L M. Atherosclerosis, 1978, 31: 259. [9] GUAN Jun-wen, XU Mei-yi(关俊文, 徐美亦). Guangdong Trace Elements Science(广东微量元素科学), 2003, 10(9): 36. [10] Fischer Peter W F. Am J. Clin. Nutr.. 1981, 34(9): 1670. [11] LI Shu-zhen(李书桢). Trace Element and Human Health(微量元素与人体健康). Huhhot: Neimenggu Press(呼和浩特: 内蒙古出版社), 1981. 28.
