1. Key Laboratory of Optical and Magnetic Resonance Spectroscopy, East China Normal University, Shanghai 200062, China 2. Shanghai Research Center of Biotechnology, Chinese Academy of Sciences, Shanghai 200233, China
Abstract：Raman spectra of human serum albumin (HSA) and HSA-3-picolylamine complex were obtained. The spectra indicate the configuration and structural transformations of HSA. The results show that the secondary structure is main α-helix, and the binding of 3-picolylamine doesn’t change the secondary structure. However, the binding changes the configuration of disulfide bonds, transforms a single tryptophan residue from exposed tryptophan residues to hydrophobic environment, and alters the microenvironment of tyrosine.
Key words：Raman spectroscopy;Human serum albumin (HSA);3-picolylamine
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