Determination of Tetracyclines by a New Spectrum Technique
GAO Hong, ZHAO Yi-bing, GUO Xiang-qun*
Department of Chemistry and the Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
Abstract：Tetracyclines are light-fluorescence substances, which cannot be detected directly by fluorometry. Herein a new spectrum method was proposed to detect tetracyclines directly by fluorometry. Under optimal conditions, the calibration graph is linear over the range 0.10-9.00 μg·mL-1 for tetracyclines, and the detection limits of tetracycline, oxytetracycline, chlortetracycline and doxycycline are 0.065, 0.067, 0.068 and 0.070 μg·mL-1,respectively.
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