1. China Three Gorges University Alan G. MacDiarmid Institute for Renewable Energy, Yichang 443002, China 2. College of Mechanical and Material Engineering, China Three Gorges University, Yichang 443002, China
Abstract：The reactions were studied for the determination of bovine serum albumin (BSA) using o-hydroxyphenylfluorone (o-HPF) as fluorescence probe reagent with fluorescence spectrophotometry. In the B-R medium, o-HPF reacts with BSA to form the stable complex compound. The reaction system of o-HPF and BSA was binary system, and the method for the determination of BSA was of good selectivity and stability and simplicity. The linear range of o-HPF fluorescence spectrophotometric method was 1.32-18.54 μg·mL-1 The quenching constant and thermodynamics constant of the fluorescence effects between BSA and o-HPF were calculated. The results showed that the non-covalent binding forces were the binding force between o-HPF and BSA.
Key words：o-hydroxyphenylfluorone (o-HPF);Bovine Serum Albumin (BSA);Fluorescence spectrophotometry
黄应平1,顾彦2,方艳芬1,刘玉良1,朱圣姬1,罗光富1. 邻羟基苯基荧光酮荧光光度法测定牛血清白蛋白[J]. 光谱学与光谱分析, 2008, 28(06): 1336-1339.
HUANG Ying-ping1,GU Yan2,FANG Yan-fen1,LIU Yu-liang1,ZHU Sheng-ji1,LOU Guang-fu1. Study on the Determination of Bovine Serum Albumin Using o-Hydroxyphenylfluorone Reagent with Fluorescence Spectrophotometry. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(06): 1336-1339.
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