估算供体-受体对荧光共振能量传递效率的研究

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摘要：在有机白光LED的研究中，能量传递效率计算方法的研究是一项很有意义的工作。作者在总结前人工作的基础上，提出了一种利用有机分子发射谱和激发谱估算有机分子荧光共振能量传递(FRET)效率相对值的新方法，这种方法的优点是数据处理过程简单，对实验仪器设备要求不高，并且不需要通常计算方法所需的荧光量子产率等参数，缺点是只能计算能量传递的相对值。利用这种方法计算了甲萘酚、溴甲酚紫、萤光素钠盐三种能量受体和能量供体核黄素在不同浓度下能量传递效率的相对值，计算结果与实验结果基本符合，验证了此方法的合理性。

关键词：供体发射谱;受体激发谱;荧光共振能量传递效率;乘积积分估算法

Abstract：The research on the method of calculating FRET efficiency is important to organic white LED research. In the present article, a new method of calculating the FRET efficiency of donor-acceptor pair by means of emission and excitation spectra was investigated. This method is easy to use to process data, needs only normal experiment equipments and doesn’t need the parameters such as fluorescence quantum yield. However it can only be used to calculate comparative result. With this method, the FRET efficiency of fluorescein sodium, α-naphthol, bromocresol purple and riboflavin with different concentrations was studied, and the results match the experimental results completely, so this method has proved to be reasonable.

Key words：Emission spectrum of donor;Excitation spectra of acceptor;Efficiency of fluorescence resonance energy transfer (FRET);Assessment method of product integral

收稿日期: 2004-08-26 修订日期: 2005-01-20

中图分类号:

O433.5

通讯作者: 王晶

引用本文:

王进，王晶^*，孙依红，孟继武. 估算供体-受体对荧光共振能量传递效率的研究[J]. 光谱学与光谱分析, 2006, 26(02): 279-281.
WANG Jin,WANG Jing^*,SUN Yi-hong,MENG Ji-wu. Investigation of Calculating the FRET Efficiency of Donor-Acceptor Pair. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(02): 279-281.

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https://www.gpxygpfx.com/CN/Y2006/V26/I02/279