Applying Patial Least-Squares Discriminant Analysis on Autofluorescence Spectra to Identify Gastric Cancer
SHI Xiao-feng1, MA Jun1*, MAO Wei-zheng2, LI Ying1, ZHENG Rong-er1, MENG Ji-wu1
1. Department of Physics, Ocean University of China, Qingdao 266071, China 2. Depatment of General Surgery, Medical College Hospital of Qingdao University, Qingdao 266003, China
Abstract：Measurement of fluorescence intensity was performed at excitation wavelength of 308 nm and emission wavelength in the range of 328-596 nm. The partial least-squares(PLS) method was used to analyze autofluorescence spectra of gastric cancer. The 42 normal samples and 42 cancer samples were taken from 42 gastric cancer patients. The normalized and centralized spectra of two kinds of samples showed similar but divergent patterns. PLS classification algorithm could differentiate cancer tissues from normal tissues with a sensitivity of 83.3%, a specificity of 95.2%, and a positive predictive value of 94.6%. We concluded therefore that the PLS method was a fast, effective choice for identification of gastric cancer.
Key words：Autofluorescence spectroscopy;Partial-least squares;Gastric cancer
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