Abstract：Two quality control methods of nuxvomica were established for mutual. By selecting the appropriate measuring wavelength or wavelength range, the contents of strychnine and brucine in nuxvomica were determined without any preliminary separation by a new rapid spectrophotometry and a multi-wavelength linear regression spectrophotometry and with a computer program. The linear range of strychnine measured was 8.0-30.0 μg·mL-1 (r=0.999 9); The linear range of brucine measured was 7.0-31.2 μg·mL-1 (r=0.999 4). The average recoveries and relative standard deviations of strychnine and brucine were 98.18%-99.82%，0.56%-1.54% and 100.5%-100.6%，0.57%-0.62%, respectively. The methods are simple, rapid and reproducible, the interference of two components with each other may be eliminated and the methods are appropriate for quality control of nuxvomica.
Key words：Nuxvomica;Strychnine;Brucine;Rapid spectrophotometry;Multi-wavelength linear regression spectrophotometry
黄喜茹，孙泽东，曹 冬, 王淑月. 马钱子的质量控制方法研究[J]. 光谱学与光谱分析, 2006, 26(02): 324-326.
HUANG Xi-ru, SUN Ze-dong, CAO Dong, WANG Shu-yue. Studies on Quality Control Methods of Nuxvomica. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(02): 324-326.
[1] LU Min-yi, LI Mei, ZHAO Su-rong, et al(陆敏仪, 李 梅，赵素荣, 等). Journal of Chinese Material Medica(中国中药杂志)，1990，15(12)：18. [2] HUANG Zhu-fang, BAI Xiao-hong(黄竹芳，白小红). Chinese Journal of Hospital Pharmacy(中国医院药学杂志)，2000, 20(3)：145. [3] HUANG Xi-ru, ZHANG Zhong-min, ZHANG Zhen-hua, et al(黄喜茹，张忠敏，张振华, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，1999, 19(3)：468. [4] HUANG Xi-ru, ZHANG Yong-shun, FU Chao-mei, et al(黄喜茹, 张永顺，付超美, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2001, 21(6)：840. [5] Pharmacopeia Committee of the Ministry of Health, the People’s Republic of China(中华人民共和国卫生部药典委员会). Pharmacopeia of the People’s Republic of China(中华人民共和国药典). Beijing: Chemical Industry Press(北京：化学工业出版社)，1995. 38.
