Establishment of Bioassay Method for Antivirus Potency of Radix Isatidis Based on Chemical Fluorometric Determination
LI Han-bing1, 2,YAN Dan1,JIN Cheng1,WANG Jia-bo1,WEI Li1, 2,XIAO Xiao-he1*,CAO Jun-ling3
1. Institute of Chinese Medicine，302 Hospital of People’s Liberation Army, Beijing 100039, China 2. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China 3. Dongzhimen Hospital, Beijing University of Traditional Chinese Medicine, Beijing 100700, China
Abstract：In the present study, the in vitro inhibitory activity of extracts from radix isatidis on neuraminidase (NA) was investigated by the chemical fluoremetic determination to establish the quality control method for antivirus action of radix isatidis. The initial study indicated that radix isatidis had obvious in vitro inhibitory activity on NA with IC50=(0.90±0.20) mg(herb)·mL-1. The correlation between logarithmic dose and reaction rate showed a “S” shape and a linear curve (linear equation, y＝8.725 9+1.216 9×log(D), R=0.999 2) when the reaction rate was converted to probit-quite similar to Tamiflu’s reaction curve, which hinted that radix isatidis had the same inhibitory function on NA as Tamiflu. According to the reaction type and the regularity of “parallel lines of qualitative effect”, the experimental condition was optimized and a statistic method was confirmed based on the principle of bioassay statistic. Then the bioassay method for antivirus potency of radix isatidis based on fluorometric determination was established. The results of bio-potency assay showed the qualitative differences of radix isatidis samples sensitively and quantitatively. Meanwhile, this method has good reproducibility with RSD=5.78% and reliability. The quality bioassay control method based on chemical fluorometric determination can reflect the pharmaco-dynamic features of Chinese medicine herb.
李寒冰1，2,鄢丹1,金城1,王伽伯1,魏丽1，2,肖小河1*,曹俊岭3 . 基于化学荧光测定的板蓝根抗病毒效价检测方法的建立[J]. 光谱学与光谱分析, 2009, 29(04): 908-912.
LI Han-bing1, 2,YAN Dan1,JIN Cheng1,WANG Jia-bo1,WEI Li1, 2,XIAO Xiao-he1*,CAO Jun-ling3 . Establishment of Bioassay Method for Antivirus Potency of Radix Isatidis Based on Chemical Fluorometric Determination. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(04): 908-912.
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