Abstract：The interaction of pulsed electric field (PEF) and bovine serum albumin (BSA) was studied by synchronous fluorescence and Raman spectroscopy. The results of synchronous fluorescence showed that pulsed electric field exerted its effects on the emission fluorescence spectrum and reduced the fluorescence intensities of the tyrosine and tryptophan side chains. The results of Raman spectroscopy verified this. These two experiments indicated that PEF exposure changed the microenvironments of the two aromatic amino acids, which were located in the active parts of BSA, and further indicated the conformational changes of the proteins, and the change in its biological functions.
Key words：Synchronous fluorescence spectroscopy;Raman spectroscopy;Pulsed electric field;Bovine serum albumin (BSA)
李乐军，陈树德*，乔登江. 脉冲电场与牛血清白蛋白相互作用的同步荧光光谱和拉曼光谱比较研究[J]. 光谱学与光谱分析, 2006, 26(01): 81-85.
LI Le-jun, CHEN Shu-de*, QIAO Deng-jiang. Synchronous Fluorescence and Raman Spectroscopy Study on the Interaction of Pulsed Electric Field (PEF) and Bovine Serum Albumin (BSA). SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(01): 81-85.
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