Synchronous Fluorimetric Determination of the Residues of Three Quinolones in Eel by Partial Least Squares
ZHANG Guo-wen1, NI Yong-nian2*
1. The Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047, China 2. Department of Chemistry, Nanchang University, Nanchang 330047, China
Abstract：In the present paper, the synchronous fluorimetric spectra of norfloxacin, lomefloxacin chlorhydric acid, and levofloxacin lactate with Δλ=190 nm in a B-R buffer medium of pH 2.87 were measured, and the partial least squares (PLS) method was applied to the quantitative resolution of the seriously overlapped fluorimetric spectra of these compounds. The linear ranges for norfloxacin, lomefloxacin chlorhydric acid, and levofloxacin lactate are 0.016-0.40 μg·mL-1, 0.01-0.336 μg·mL-1 and 0.01-0.336 μg·mL-1, respectively. The limits of detection are 0.012 6 μg·mL-1 for norfloxacin, 0.006 μg· mL-1 for lomefloxacin chlorhydric acid, and 0.007 2 μg·mL-1 for levofloxacin lactate. The analytical results by PLS were compared with principal components regression (PCR) and classical least squares (CLS), and this method was applied to the determination of these three compounds in eel samples with satisfactory results.
Key words：Partial least squares;Synchronous fluorimetry;Quinolones
张国文1，倪永年2*. 偏最小二乘-同步荧光光谱法同时测定鳗鱼组织中三种喹诺酮药物残留量[J]. 光谱学与光谱分析, 2006, 26(01): 113-116.
ZHANG Guo-wen1, NI Yong-nian2*. Synchronous Fluorimetric Determination of the Residues of Three Quinolones in Eel by Partial Least Squares. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(01): 113-116.
[1] Bailac S, Ballesteros O, Jimenez-Lozano E, et al. J. Chromatography A, 2004, 1029(1-2): 145. [2] LI Jun-suo, QIU Yue-ming, WANG Cao(李俊锁, 邱月明, 王 超). The Analysis of the Animal Drug Residue(兽药残留分析). Shanghai: Shanghai Scientific and Technical Press(上海: 上海科学技术出版社), 2002. [3] Gigosos P G, Revesado P R, Cadahia O, et al. J. Chromatography A, 2000, 871: 31. [4] Ramos M, Aranda A, Garcia E, et al. J. Chromatography B, 2003, 789: 373. [5] Manceau J, Gicquel M, Laurentie M, et al. J. Chromatography B, 1999, 726: 175. [6] XU Qing-qin, DU Li-ming, WANG Jing-ping(许庆琴, 杜黎明, 王静萍). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2002, 22(3): 444. [7] DU Li-ming, CAO Xi-min, WU Hong-yan(杜黎明, 曹玺珉, 吴红艳). Chinese Journal of Analytical Chemistry(分析化学), 2003, 31(11): 1330. [8] NI Yong-nian(倪永年). The Applications of Chemometrics in Analytical Chemistry(化学计量学在分析化学中的应用). Beijing: Science Press(北京: 科学出版社), 2004. [9] ZHANG Yong, HUANG Xian-zhi, XU Jin-gou, et al(张 勇, 黄贤智, 许金钩, 等). Chinese Journal of Analytical Chemistry(分析化学), 1994, 22(5): 445. [10] Otto M, Wegscheider W. Analytical Chemistry, 1985, 57: 63.
