Spectral Analysis and Study of the Microsome Cytochrome P450 Aromatase of Thais clavigera
HAN Ya-li1,DENG Rui-peng2,ZHOU Xiao-peng2
1. Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510090, China 2. Department of Biology, Shantou University, Shantou 515063,China
Abstract:The microsomes were prepared from the viscera masses of Thais clavigeras. It is well known that there are some main components in microsome cytochrome P450 aromatase, including cytochrome P450, cytochrome b5 and NADPH-cytochrome P450 reductase. So the contents of cytochrome P450 and cytochrome b5, and the activity of NADPH-cytochrome P450 reductase were determined by Shimadzu UV Visible spectrophotometer. And the microsomal proteins were analysed by SDS-PAGE. All the results indicated that there exists P450 arom in the T. clavigeras.
韩雅莉1, 邓瑞鹏2, 周小朋2. 疣荔枝螺微粒体细胞色素P450酶系的光谱分析[J]. 光谱学与光谱分析, 2006, 26(01): 130-133.
HAN Ya-li1,DENG Rui-peng2,ZHOU Xiao-peng2 . Spectral Analysis and Study of the Microsome Cytochrome P450 Aromatase of Thais clavigera. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(01): 130-133.