Determination of Nutrient Elements in Transgenic Insect-Resistant Cotton Tissues by Three Different Spectroscopical Methods
SUN Cai-xia1, ZHANG Yu-lan2, SUN Yu-quan1, YANG Lei1, WANG Jie1, CUI Zhen-bo1
1. Science College, Northeastern University, Shenyang 110004, China 2. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
Abstract：In order to find out the effects of exogenous genes, such as Bt and Bt coupled with CpTI, on nutrition metabolism in transgenic plants, totally eleven types of nutrient elements in transgenic Bt (Z30) and Bt-CpTI (CCRI41 and SGK321) cotton were determined using methods of flame atomic absorption spectroscopy, flame atomic emission spectroscopy and spectrophotometry at flowering stage and boll-opening stage. The results showed that the chemical composition of plant nutrition in transgenic insect-resistant cotton differed in comparison with non-transgenic cotton counterparts related to varieties, tissues and stages. The content of total N in transgenic cotton changed most significantly. Especially, it increased by 21% for transgenic Bt cotton Z30 compared to non-transgenic cotton Z16. These changes in total N content were probably caused by both transgenes expression in transgenic cotton and other processes not studied in this experiment. The content of Mg, Na and Cu in transgenic cotton varied significantly only in some certain varieties or tissues. It was unobvious how the incorporation of transgenes impacted on the content of organic C, total P, total S, K, Ca, Fe and Zn in transgenic cotton. The authors speculated that there were no significant changes in utilization and accumulation of these nutrient elements between transgenic insect-resistant cotton and their non-transgenic cotton counterparts (Z16, CCRI23 and SY321, respectively).
Key words：Transgenic insect-resistant cotton;Spectroscopy methods;Nutrient elements
孙彩霞1，张玉兰2，孙玉全1，杨 磊1，王 杰1，崔振波1 . 三种不同光谱学方法测定转基因抗虫棉组织营养元素含量[J]. 光谱学与光谱分析, 2009, 29(11): 3038-3041.
SUN Cai-xia1, ZHANG Yu-lan2, SUN Yu-quan1, YANG Lei1, WANG Jie1, CUI Zhen-bo1 . Determination of Nutrient Elements in Transgenic Insect-Resistant Cotton Tissues by Three Different Spectroscopical Methods . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(11): 3038-3041.
[1] LIU Qian, ZHU Xin-quan(刘 谦, 朱鑫泉). Biosafety(生物安全). Beijing: Science Press(北京: 科学出版社), 2001. 28. [2] SUN Cai-xia, WU Zhi-jie, CHEN Li-jun(孙彩霞, 武志杰, 陈利军). Acta Ecologica Sinica(生态学报), 2004, 24(4): 798. [3] Cellini F, Chesson A, Colquhoun I, et al. Food and Chemical Toxicology, 2004, 42: 1089. [4] Motavalli P P, Kremer R J, Fang M, et al. Journal Environmental Quality, 2004, 33(3): 816. [5] Zwahlen C, Hilbeck P G, Nentwig W. Molecular Ecology, 2003, 12(3): 765. [6] LU Ru-kun(鲁如坤). Methods of Chemical Analysis in Soil and Agriculture(土壤农业化学分析方法). Beijing: Agricultural Science and Technology Press(北京：中国农业科技出版社), 1999. 252. [7] LI Qing-fang, MA Cheng-cang(李清芳, 马成仓). Scientia Agricultura Sinica(中国农业科学), 2003, 36(6): 726. [8] Conner A J, Jacobs J M E. Mutation Research/Genetic Toxicology Environmental Mutagenesis, 1999, 443: 223. [9] DONG Zhi-qiang, HE Zhong-pei, ZHAI Xue-jun (董志强, 何钟佩, 翟学军). Cotton Science(棉花学报), 2000, 12(30): 113. [10] Bruns H A, Abel C A. Agronomy Journal, 2003, 95: 207. [11] SUN Cai-xia, ZHANG Li-li, WU Qiong, et al (孙彩霞, 张丽莉, 吴 琼, 等). Chinese Journal of Ecology(生态学杂志), 2007, 26(2): 187. [12] WANG Bao-min, LI Zhao-hu, LI Bin, et al(王保民, 李召虎, 李 斌, 等). Journal of Agricultural Biotechnology(农业生物技术学报), 2002, 10(3): 2l5. [13] Escher N, Kach B, Nentwig W. Basic Applied Ecology, 2000, 1(2): 161.
