Abstract：The interaction of tetrandrine with bovine serum albumin was studied by fluorescence spectra and ultra-violet spectra. The results showed that tetrandrine could quench the intrinsic fluorescence of BSA. Both static quenching and non-radiation energy transfer were the main reasons for the fluorescence quenching. The quenching constants KSV at different temperatures were determined using Stern-Volmer equation. The KSV were 1.26×104 L·mol-1 (300 K), 1.17×104 L·mol-1 (310 K) and 1.12×104 L·mol-1 (320 K). According to the Frster theory of non-radiation energy transfer, the binding distances (r) were 3.24 nm (300 K), 3.31 nm (310 K) and 3.50 nm (320 K). The binding constants (KA) between tetrandrine and BSA (300 K: 1.52×105 L·mol-1; 310 K: 2.03×105 L·mol-1; 320 K: 2.89×105 L·mol-1) and thermodynamic parameters were also obtained. The thermodynamic parameters indicated that the interaction of tetrandrine and BSA was driven mainly by hydrophobic force. Results of synchronous fluorescence spectrum showed that the binding could cause conformational changes of BSA.
吴秋华，宋双居，王 春，王 志*. 粉防己碱与牛血清白蛋白相互作用的研究[J]. 光谱学与光谱分析, 2009, 29(11): 3088-3091.
WU Qiu-hua, SONG Shuang-ju, WANG Chun, WANG Zhi*. Study on the Interaction of Tetrandrine and Bovine Serum Albumin. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(11): 3088-3091.
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