Study on Extractive Spectrophotometry for Determination of Amoxicillin with Ionic Associate
DUAN Ya-li1, DU Li-ming2*，CHEN Cai-ping1
1. College of Chemistry and Material Science, Shanxi Normal University, Linfen 041004, China 2. Center of Analysis and Testing, Shanxi Normal University, Linfen 041004, China
Abstract：A method of extractive spectrophotometry has been proposed for the determination of amoxicillin(AMO). The ion pairs complex of amoxicillin with methylene blue (MB) can be extracted into 1,2-dichloroethane at pH 3.3 and has absorption maximum at 657 nm. The ratio of amoxicillin to methylene blue in the ion pairs complex is 1∶1. The apparent molar obsorptivity of ion pairs complex is 5.0·104 L·mol-1·cm-1 at 657 nm. The calibration graph generated is linear over the range of 0.4-6.8 mg·L-1 drug in solution with a correlation of 0.998 9. The detection limit is 0.01 mg·L-1 and the recovery of AMO is 97.5%-101.3%. The present method has been applied to the determination of amoxicillin in pharmaceutical formulations and urine samples with satisfactory results.
段亚丽1，杜黎明2*，陈彩萍1 . 离子对缔合物萃取分光光度法测定阿莫西林[J]. 光谱学与光谱分析, 2005, 25(11): 1865-1867.
DUAN Ya-li1, DU Li-ming2*，CHEN Cai-ping1 . Study on Extractive Spectrophotometry for Determination of Amoxicillin with Ionic Associate . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(11): 1865-1867.
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