1. College of Chemistry and Materials, Fujian Normal University, Fuzhou 350007, China 2. The First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, China 3. State Key Laboratory for Structure Chemistry, Chinese Academy of Sciences, Fuzhou 350003, China
Abstract：Octa-carboxylic phthalocyanine aluminium (AlPc(COOH)8) was used as fluorescent probe of infrared region. The interaction of octa-carboxylic phthalocyanine aluminium (AlPc(COOH)8) and bovine serum albumin(BSA) was studied by UV/Vis and fluorescence spectra methods. The binding constant KA and n of phthalocyanine aluminium with BSA were determined. The results were K=5.74×105, n=5.7 and K=3.51×105,for these two methods respectively. The same results by using two different analytical methods were obtained. Besides, hemin chloride(HE), ibuprofen(IB) and L-tryptophan(TRP) were used as probes, and the effects of these probes on the spectra of AlPc(COOH)8)-BSA were studied by competitive binding method. The result indicated that, by adding HE into the AlPc(COOH)8)-BSA system, obvious spectral change of the system was observed, while adding TRP and IB caused no spectral changes. The binding site of octa-carboxylic phthalocyanine aluminium on the BSA was found to be at theIsite by competitive binding method.
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