三丁基锡(TBT)化合物与牛血清白蛋白(BSA)的相互作用

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摘要：通过紫外、荧光和圆二色(CD)光谱，研究了船体防污漆的防污成分三丁基锡(TBT)化合物与牛血清白蛋白(BSA)的相互作用，考察了浓度、酸度和有机溶剂等因素的影响。结果表明，TBT与BSA的相互作用是双重的，既有TBT中丁基基团的疏水作用，又有锡离子与BSA的配位作用，使BSA内部的色氨酸和酪氨酸等芳香氨基酸残基裸露，导致BSA二级结构破坏，α-螺旋含量减少和构象改变。

关键词：三丁基锡(TBT);牛血清白蛋白(BSA);相互作用

Abstract：Tributyltin (TBT) compound is one of the main components of antifouling paint for boats and ships. The interaction of TBT compound and bovine serum albumin (BSA) was investigated by ultraviolet, fluorescence and circular dichroism (CD) spectra. The influences of concentration, acidity and organic solvent were also studied. The results showed that the interaction of TBT and BSA was dual, showing not only the hydrophobic actions of butyl groups but also the coordination action of tin cation with BSA molecule, which resulted in the exposures of aromatic amino acids residues of tryptophane and tyrosine in BSA molecule, the destruction of BSA secondary structure, the decrease in α-helix content, and the transformation of the conformation.

Key words：Tributyltin (TBT);Bovine serum albumin (BSA);Interaction

收稿日期: 2005-10-16 修订日期: 2006-01-18

中图分类号:	Q523
	O657.3

通讯作者: 臧树良 E-mail: zzhly@163.com

引用本文:

张朝红¹,董殿波¹,赵哲¹,臧树良^1,2*,陈中林¹,王杰¹,张华春¹ . 三丁基锡(TBT)化合物与牛血清白蛋白(BSA)的相互作用[J]. 光谱学与光谱分析, 2007, 27(02): 309-312.
ZHANG Zhao-hong¹,DONG Dian-bo¹,ZHAO Zhe¹,ZANG Shu-liang^{1, 2*},CHEN Zhong-lin¹,WANG Jie¹, ZHANG Hua-chun¹. Interaction of Tributyltin (TBT) Compound and Bovine Serum Albumin (BSA). SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(02): 309-312.

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