Abstract：Two novel complexes of [Cu(HAsp)ImH2O]SO4·4H2O and [Cu(Asp)Im(OH)]·4H2O (HAsp=Aspartic acid molecule, Asp=Aspartic acid ion, Im=Imidazole) were synthesized and characterized by elemental analysis, IR spectroscopy and TG-DTA. The interactions of the complexes with sperm DNA were studied by electronic absorption and fluorescence spectra. Results showed that the reactions of the two complexes with DNA are obviously different: for [Cu(HAsp)ImH2O]SO4·4H2O, it is intercalation companied by electrostatic effect, while [Cu(Asp)Im(OH)]·4H2O mainly cooperates with the nitrogen atom of the DNA base pair, which induces the breakage of DNA double helix. The reasons for these differences in their DNA binding modes were also discussed.
[1] Laura Santagostini, Michele Gullotti, Roberto Pagliarin, et al. Tetrahedron: Asymmetry, 1999, 10(1): 281. [2] WANG Zhong-ming, ZHOU Zhi-fen, LIN Hua-kuan, et al(汪中明, 周志芬, 林华宽, 等). Chinese Journal of Inorganic Chemistry(无机化学学报), 2000, 16(3): 503. [3] Liu Jie, Zhang Ti-xiang, Lu Tongbu, et al. Journal of Inorganic Biochemistry, 2002, 91: 269. [4] LI Hong, LE Xue-yi, WU Jian-zhong, et al(李 红, 乐学义, 吴建中, 等). Acta Chimica Sinica(化学学报), 2003, 61(2): 245. [5] Hirohama Tomoya, Yuko Kuranuki, Ebina Ebina, et al. Journal of Inorganic Biochemistry, 2005, 99: 1205. [6] ZHOU Qing-hua, YANG Pin(周庆华, 杨 频). Acta Chimica Sinica(化学学报), 2005, 63: 71. [7] ZHANG Fang, ZHANG Qian-qian, ZHU Chen-jian, et al(张 芳，张前前，祝陈坚, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(9): 1439. [8] WANG Y Y, SHI Q, SHi Q Z. Polyhedron, 1999, 18(5): 2009. [9] Török I, Surdy P. Journal of Inorganic Biochemistry, 1998, 71: 7. [10] ZHAO Guang-chao, ZHU Jun-jie, CHEN Hong-yuan(赵广超, 朱俊杰, 陈洪渊). Chemical Journal of Chinese Universities(高等学校化学学报), 2003, 24: 414. [11] LI Rui-ping, FAN Qiong-fang, GUO Jian-xiong(李瑞萍, 范琼芳, 郭建雄). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(2): 288. [12] Laila H A R, Luigi P B, Daniele C. Polyhedron 1996, 15(11): 1783. [13] Latif A Abuhijleh, Clifton Woods. Inorganic Chemistry Communications, 2001, 4(3): 119. [14] Nakamoto K. Infrared and Raman Spectra of Inorganic and Coordination Compounds, 3rd ed., New York: John Wiley & Sons, 1978, 214. [15] Thomas N S, William E A, White S P. Journal of Inorganic Biochemistry, 1995, 59: 659. [16] Han Gaoyi, Yang Pin. Journal of Inorganic Biochemistry, 2002, 91: 230. [17] YANG Pin, GUO Mao-lin, YANG Bin-sheng(杨 频, 郭茂林, 杨斌盛). Chinese Science Bulletin(科学通报), 1994, 39: 997. [18] GUO Mao-lin, YANG Pin, YANG Bin-sheng, et al(郭茂林，杨 频，杨斌盛, 等). Chinese Science Bulletin(科学通报), 1995, 40: 1187.
