蛋白质三元复合物空间定向作用机理的研究——BSA/SDS/天青A反应体系

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摘要：应用光谱探针技术研究了牛血清白蛋白(BSA)/十二烷基硫酸钠(SDS)/天青A(AA)相互作用的吸收光谱，考察了SDS/BSA摩尔比对BSA/SDS/AA反应体系吸收光谱的影响。研究结果表明，BSA-SDS-AA三元复合物产生新的光谱吸收峰以及变色反应，主要是由于结合态AA分子空间定向作用形成的聚集体聚集程度不同所造成的。

关键词：牛血清白蛋白;十二烷基硫酸钠;天青A;空间定向作用;光谱探针

Abstract：The interaction of bovine serum albumin (BSA)，sodium dodecyl sulphate (SDS) and Azur A(AA) was studied by spectral probe. The influence of the molar ratio of SDS/BSA on the absorption spectra of BSA-SDS-AA complex was investigated. It was found that the new absorption peak and color changes generated by BSA-SDS-AA complex arose from the difference in the spatial orientation aggregative degree of AA binding on BSA-SDS-AA complex.

Key words：Bovine serum albumin;Sodium dodecyl sulphate;Azur A;Spatial orientation interaction;Spectral probe

收稿日期: 2003-06-15 修订日期: 2004-11-21

中图分类号:

O657.3

通讯作者: 曹稳根

引用本文:

曹稳根^{1 , 2}，焦庆才¹, 刘茜¹ . 蛋白质三元复合物空间定向作用机理的研究——BSA/SDS/天青A反应体系[J]. 光谱学与光谱分析, 2005, 25(09): 1478-1481.
CAO Wen-gen^{1, 2}, JIAO Qing-cai¹,LIU Qian¹ . Spatial Orientation Interaction Mechanism of Three Component Complex of Protein ——BSA/SDS/Azur a System. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(09): 1478-1481.

链接本文:

https://www.gpxygpfx.com/CN/Y2005/V25/I09/1478

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