Abstract：The adsorption orientation and adsorption characteristics of aliphatic-nonpolar amino acids represented by L-methionine on silver nanoparticles were studied by FT-surface-enhanced Raman spectroscopy (FT-SERS) method with different pH and concentrations. According to the experiments，in order to get the best FT-SERS effects，the optimal concentration range of the L-methionine on silver sol is between 10-3 mol·L-1-10-4 mol·L-1. The FT-SERS effects and adsorption model change with pH;The SERS are poor when pH is near the isoelectric point or over 11 or 3. The interaction of L-methionine with Ag nanoparticles is the physical and chemical adsorption by NH3,COO-，S，which could also be effected by pH. From the results and previous reports on this aspect, the general rules of the adsorption model and the orientation of the aliphatic-nonpolar amino acids on silver nanoparticles were speculated, which would be useful for the study of amino acids, protein, peptide, enzyme etc.
黎司,周光明*,杨大成,虞丹尼,彭红军,伍辛军,王宁. FT-SERS研究非极性R侧链氨基酸在银胶体系中的吸附状态[J]. 光谱学与光谱分析, 2007, 27(04): 711-715.
LI Si, ZHOU Guang-ming*,YANG Da-cheng, YU Dan-ni, PENG Hong-jun, WU Xin-jun, WANG Ning. Study on Adsorption of Nonpolar R-Side Amino Acids on Silver Nanoparticles by FT-SERS. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(04): 711-715.
[1] Nie S，Emory S R. Science, 1997, 275：1102. [2] Kneipp B，et al. Chem. Euv. J.，1997, 3：363. [3] Kneipp K，et al. Chimia, 1999, 53: 35. [4] Campion A，Kambhampati P. J. Chem. Soc. Rev.，1998，27(4)：241. [5] YU Zi-ran(于自然). Modern Biochemistry(现代生物化学). Beijing: Chemical Industry Press(北京: 化学工业出版社)，2001. [6] KE Wei-zhong，WU Jian-zhong(柯惟中，吴缄中). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(5)：551. [7] ZHU Zhi-liang，GAO Jun-ying，LI Feng-ting, et al(朱志良，郜俊影，李风亭, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(1)：68. [8] Abello L，Genet F，Nigretto J M, et al. Surface Sci., 1989，215(1-2) ：158. [9] Lee H，Kim M S，Suh S W. Journal of Raman Spectroscopy, 1991，22：91. [10] Lee P C，Meisel D. J. Phys. Chem.，1982，86：3391. [11] Colthup N B，Daly L H，Willely S E. Introduction to Infrared and Raman Spectroscopy. New York：Academic Press，1990. [12] Brown I D. Acta. Cryast. A，1976，32: 24. [13] Dollish F R，Fateley W G. Bentley Characteristic Raman Frequencies of Organic Compounds. New York：Wiley，1973. [14] Mary M B，et al. Spectrochimica Acta, Part A, 2004，60: 2643. [15] LIN Han-jie(林汉杰). Journal of Instrumental Analysis(分析测试学报)，1994，13(2): 19. [16] Suh J S，Moskovits M. J. Am. Chem. Soc.，1986, 108: 4711. [17] Chumanov G D, Efremov R G, Nabive I R. Journal of Raman Spectroscopy，1990，21: 43. [18] Nabive I R，Chumanov G , Efremov R G. Journal of Raman Spectroscopy，1990，21: 49. [19] QU Jin-rong, YE Tian-xiu(瞿金蓉，叶天秀). Journal of Hubei Polyteohnic University(湖北工学院学报), 2000，15(2): 5. [20] Greenler R G, Snider D R, Witt D，et al. Surface Sci.，1982，118: 415. [21] Moskovits M, Suh J S. J. Phys. Chem.，1984，88: 5526. [22] Moskovits M, Suh J. S. J. Phys. Chem.，1988, 92: 6327. [23] Demuth J E，Christmann K, Sanda P N. Chem. Phys. Lett.，1980，76：201. [24] JIANG Shuo-jian, DING You-jun, LI Ming-qian(蒋硕健，丁有骏，李明谦). Organic Chemistry(有机化学). Beijing：Peking University Press(北京：北京大学出版社)，1989. [25] Song O K，Yoon M J. Journal of Raman Spectroscopy, 1991, 22: 729. [26] Chumanov G D，Efremov R G，Nabive I R. Journal of Raman Spectroscopy，1990，21：43. [27] ZHOU Hai-hui, WU De-yin, HU Jian-qiang, et al(周海辉，吴德印，胡建强, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005，25(7): 1068.
