Simultaneous Determination of Five Banned Sartan Compounds in Antihypertensive Health Food Using Thin-Layer Chromatography Combined With Surface-Enhanced Raman Spectroscopy (TLC-SERS)
HUANG Li-jun1, ZHUANG Shun-qian2, FENG Yong-wei1, YANG Fang-wei2, SUN Zhen1, XIE Yun-fei2*, XUE Qing-hai1
1. Wuxi Food Safety Inspection and Test Center, Wuxi 214412, China
2. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Abstract：Due to the current unhealthy diet structure and lifestyle habits, as well as the aging population and other factors, the incidence of chronic diseases such as hypertension is gradually increasing, and people gradually favor antihypertensive health food. However, in order to improve the efficacy of healthy food, there are unscrupulous businessmen in the market who illegally add antihypertensive drugs, especially sartans antihypertensive drugs, to healthy food. Based on the above phenomenon, this study established a Thin-Layer Chromatography (TLC) and Surface-enhanced Raman spectroscopy (SERS) method to investigate five possible prohibited ingredients of sartans (Olmesartan Medoxomil, Irbesartan, Losartan potassium, Telmisartan and Valsartan) in antihypertensive health food products. In this study, we investigated the optimal conditions for the SERS detection of five drugs, compared the effects of TLC spreaders with different ratios of different components on the separation of the five drugs. We successfully obtained the separation system of cyclohexane-ethyl acetate-methanol-glacial acetic acid=53∶35∶12∶0.25 (v/v/v/v) after extensive formulation tests. After determining the exact conditions of this experiment, the preliminary separation of drug standard solution, mock positive sample solution and real sample solution was carried out by using TLC separation technique. After separation, gold gel was added dropwise at the spot in situ with different ratio shift values on the thin layer plate for SERS detection. The SERS spectra of drug standard solution as well as mock positive sample solution at the spot in situ were collected, compared and determined. The results showed that the five prohibited additive ingredients of sartans could be clearly separated by TLC, their SERS spectra were consistent and reproducible, and the qualitative analysis could be performed quickly according to their respective characteristic peaks, with detection limits between 0.1 and 1 mg·kg-1. The TLC-SERS method established in this study is simple and rapid. It can provide a reference for the simultaneous on-site rapid detection of prohibited added ingredients in health food.
Key words：Antihypertensive health food;Sartans;TLC-SERS;Simultaneous identification
[1] YANG Xue, DOU Xiao-feng, MA Jie, et al(杨 雪, 窦晓凤, 马 捷, 等). China Food Safety Magazine(食品安全导刊)，2022，(4): 164.
[2] Mills K T, Stefanescu A, He J. Nature Reviews Nephrology, 2020, 16(4): 223.
[3] Writing Group of 2018 Chinese Guidelines for the Management of Hypertension(2018年中国高血压防治委员会，高血压联盟). Chinese Journal of Cardiovascular Medicine(中国心血管杂志), 2019, 24(1): 24.
[4] YANG Hu-qing, SUN Jian, JI Shen(杨胡青, 孙 健, 季 申). China Food & Drug Administration Magazine(食品安全导刊), 2022，(3): 88.
[5] LI Ying, YANG Yin(李 莹, 羊 银). China Food Additives(中国食品添加剂), 2021, 32(4): 112.
[6] TIAN Tian, QIN Yan-shu, ZHAO De-en, et al(田 甜, 覃艳淑, 赵德恩, 等). Journal of Food Safety & Quality(食品安全质量检测学报), 2021, 12(3): 1200.
[7] YUE Lin, TAN You-neng, SU Fang, et al(岳 琳, 谭有能, 粟 芳, 等). Chinese General Practice Nursing(全科护理), 2012, 10(15): 1406.
[8] ZHANG Zhong-hu, LI Jun, XIE Qiang-sheng, et al(张中湖, 李 军, 谢强胜, 等). Chinese Journal of Pharmaceutical Analysis(药物分析杂志), 2019, 39(3): 467.
[9] CHEN Lin, WEN Jia-xin, QI Chun-yan, et al(陈 林, 温家欣, 齐春艳, 等). Journal of Instrumental Analysis(分析测试学报), 2016, 35(8): 937.
[10] XU Wen-feng, JIN Peng-fei, XU Shuo, et al(徐文峰, 金鹏飞, 徐 硕, 等). Chinese Journal of Pharmaceutical Analysis(药物分析杂志), 2019, 39(7): 1295.
[11] ZHAO Yi-liang, ZHAO Xing-xin, TIAN Mei, et al(赵义良, 赵兴鑫, 田 梅, 等). Journal of Food Safety & Quality(食品安全质量检测学报), 2021, 12(2): 452.
[12] HU Jia-yong, PENG Qing-zhi, ZHANG Li, et al(胡家勇, 彭青枝, 张 莉, 等). Journal of Food Safety & Quality(食品安全质量检测学报), 2021, 12(1): 7.
[13] ZHENG Xiao-jun, FANG Huai-fang, LIU Yuan-feng, et al(郑孝军, 方怀防, 刘元凤, 等). Chemical Research and Application(化学研究与应用), 2021, 33(3): 548.
[14] Shi Siqian, Yu Hang, Yang Fangwei, et al. Food Bioscience, 2022, 48: 101755.
[15] Yang Fangwei, Wang Cheng, Yu Hang, et al. Food Bioscience, 2022, 49: 101842.
[16] Singh J S, Khan M S, Uddin S. Polymer Bulletin, 2023, 80: 3055.
[17] Zhang Fu, You Jinling, Tang Xiaohui, et al. Journal of Molecular Liquids, 2023, 387: 122485.
[18] Zayed M A, El-Desawy M, Eladly A A. Computational Biology and Chemistry, 2018, 76: 338.
[19] SHI Si-qian, YANG Fang-wei, YAO Wei-rong, et al(施思倩, 杨方威, 姚卫蓉, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2021, 41(12): 3759.
[20] ZHANG Yan, YANG Zhao, ZHU Run-jie, et al(张 燕, 杨 钊, 朱润洁, 等). Chinese Pharmaceutical Journal(中国药学杂志), 2012, 47(2): 141.
