Study on the Application of Methybenzeneazosalicylfluorone to Spectrophotometric Determination of Microelement Molybdenum Modality Distributions in Soy Hydrolysate Hydrolyzed by Enzymes
LIU Hui-zhen,LI Zai-jun*
College of Chemical and Materials Engineering, Southern Yangtze University, Wuxi 214036, China
Abstract：A new method has been developed for spectrophotometric determination of molybdenum modality distributions in soy hydrolysate hydrolyzed by enzymes, based on the study on the color reaction of methybenzeneazosalicylfluorone (abbreviated as MBASF) with molybdenum(Ⅵ). In hydrochloric acid medium, MBASF reacts with molybdenum(Ⅵ) sensitively to form a stable red complex, its apparent molar absorptivity is 1.54 L·mol-1·cm-1,and 0-12 μg of molybdenum obeys Beer’s law in 25 mL of solution. Besides normal metal ions, protein, peptide and amino acid also have high tolerance limits. Soy hydrolysate hydrolyzed by enzyme (A) was precipitated at its isoelectric point, and the filtrate (B) was obtained. Subsequently, B was transferred to the chromatogram column with D301R resin. The column was purged by using 20 mL of HAc-NaAc(pH 3.6) and NH4Cl-NH3·H2O buffer solution(pH 9) orderly and the effluent solutions C and D were obtained. A, B, C and D solutions were used for the spectrophotometric determination of total molybdenum, hydrolyzed molybdenum, chelated molybdenum by peptide or amino acid, and dissociative molybdenum, respectively.
刘慧珍,李在均*. 对甲苯偶氮水杨基荧光酮应用于大豆酶解液中钼的形态分布研究[J]. 光谱学与光谱分析, 2007, 27(05): 957-960.
LIU Hui-zhen,LI Zai-jun*. Study on the Application of Methybenzeneazosalicylfluorone to Spectrophotometric Determination of Microelement Molybdenum Modality Distributions in Soy Hydrolysate Hydrolyzed by Enzymes. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(05): 957-960.
[1] YANG Ke-di(杨克敌). Microelement and Healthy(微量元素与健康). Beijing: Science Press(北京: 科学出版社), 2003. 219. [2] ZHAO Ai-dong, ZHAI Xue-liang, LIU Jing-ze(赵爱东, 翟学良, 刘敬泽). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(11): 189. [3] ZHU Xiao-mei, HAN Sen(朱小梅，韩 森). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(5): 783. [4] NI Rui-xing, GUO Zhi-bin, LIU Xing-lian(倪瑞星, 郭志斌, 刘杏恋). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(4): 776. [5] ZHU Qing-ren, SUN Deng-ming, LI Hai-yan(朱庆仁, 孙登明, 李海燕). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2002, 22(1): 107. [6] Di J W, Yang T Y. Talanta, 2003, 61(2): 165. [7] CHEN Guan-hua, SUN Han-wen(陈冠华，孙汉文). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 1997, 17(6): 49. [8] ZHANG Qi-wei, XIONG Chun-hua(张启伟，熊春华). Chinese Nonferrous Metals(有色金属), 2005, 57(3): 58. [9] Li Z J, Yang Y L, Tang J, et al. Journal of Food Composition and Analysis, 2005, 18: 561. [10] Sook Y Kim. J. Agsic. Food Chem.，1990, 38: 651.
