Second Derivative FTIR Spectroscopic Study of Bacillus Cereus
WANG Jia-rong1,MA Mei-hu1*,ZHOU Chuan-yun1,NIE Ming2
1. Faculty of Food Science and Technology，Hunan Agricultural University，Changsha 410128，China 2. College of Life and Environment Science，Shanghai Normal University，Shanghai 200234，China
Abstract：Some particular cell components of intact Bacillus cereus could be detected and identified by Fourier transform infrared spectroscopy (FTIR). FTIR were collected directly, then the FTIR spectra of the samples were converted into second derivative spectra. Typical marker bands were used to identify these bacterial cell components such as capsules, endospores or storage materials. Capsules were detected in cell by a strong amide band near 1 654 cm-1 typical for α-helical structures and by strong carboxylate stretching vibrations(≈1 601 cm-1 and ≈1 403 cm-1,respectively). The formation of endospores was discovered using marker bands for dipicolinic acid (located at ≈1 617 cm-1,≈1 372 cm-1 and ≈1 569 cm-1). Spectra of this strain showed expression of poly-β-hydroxybutyric acid granules, capsules and endospores simultaneously. These bacterial cell components can be identified by second derivative FTIR spectroscopy which can distinguish the overlap spectrum. And that offered some referenced information for molecular biology and cellular biology.
