Spectral Analysis and Synthesis of Artificial Antigen of the Organophosphors Pesticide Methamidophos
WANG Xiang-hong1,2,DUAN Zhen-juan1,SONG Shi-ying1,WANG Shuo1*
1. Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Food Science and Biologic Technology College, Tianjin 300457, China 2. Agricultural University of Hebei, Food Science and Technology College, Baoding 071001, China
Abstract：In the present paper, a hapten of methamidophos was synthesized and conjugated with KLH by active ester method, thus the first artificial antigen was obtained. By diazotization method methamidophos conjugated with BSA, and the second artificial antigen was obtained. The synthesized haptens were characterized by MS, IR and 1H NMR, and the two artificial antigens were determined by the method of IR spectrum. The result implied that both the artificial antigens have absorbance peaks of hapten and protein, indicating that they were prepared successfully. This could provide evidence that the method of IR spectrum can be used to determine whether the artificial antigens are synthesized successfully.
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