Investigation of Novel Method for Detecting Vanillin Based on X-Ray Diffraction Technology
SHI Zhi-feng1, 2, LIU Jia2, XIAO Juan2, ZHENG Zhi-wen1*
1. National Engineering Research Center for Tissue Restoration and Reconstruction and Key Laboratory of Biomedical Engineering of Guangdong Province，South China University of Technology，Guangzhou 510006，China
2. Medical Devices Research and Testing Center, South China University of Technology，Guangzhou 510006，China
Abstract：Milk powder is the main nutritional product for infants when they are not breast-fed. The frequent occurrence of melamine, vanillin and other additives has aroused great concern for society. How to more quickly detect and efficiently identify the adverse substances in milk powder is a research hotspot in the field of dairy product safety at home and abroad, which has very important practical significance to ensure the growth and health of infants. Due to a large number of absorption peaks in IR and Raman spectra of starch and vanillin, the overlap region of spectral signals between substrate and additive was wide, and the signal-to-noise ratio was low, which led to serious speak overlap. The more sensitive the instrument is, the more difficult it is to detect and screen. In this study, a non-destructive, rapid and highly sensitive method for determining vanillin as a food additive was established based on X-ray diffraction (XRD). The matrix materials, such as starch and protein of food dairy products, did not have complex diffraction patterns under XRD. Therefore,the detection method of vanillin based on XRD eliminated the background interference of various substrates in food and dairy products.The doping of vanillin could be quickly identified and qualitatively identified by combining the fingerprint characteristics of XRD patterns. The results showed a sharp diffraction peak at 13.03° with good peak shape and high response value in the diffraction pattern after doping vanillin, which would be a characteristic fingerprint peak for rapid detection of vanillin. Compared with gas chromatography and electrophoresis, this method could be more convenient, rapid, accurate in qualitative confirmation to obtain more reliable results and strong applicability while removing matrix influence by direct injection and fine scanning. In this study, the minimum doping amount of vanillin was 202 μg·g-1. The method can be used to rapidly detect vanillin in commercial flavoring foods, and the sensitivity can meet the requirements of recommended limit and excessive application.
石志锋，刘 佳，肖 娟，郑志雯. 基于X射线衍射的香兰素快速检测技术研究[J]. 光谱学与光谱分析, 2023, 43(05): 1563-1568.
SHI Zhi-feng, LIU Jia, XIAO Juan, ZHENG Zhi-wen. Investigation of Novel Method for Detecting Vanillin Based on X-Ray Diffraction Technology. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1563-1568.
[1] CHEN Da, ZOU Jian, TAN Zong, et al（陈 达，邹 建，谭 棕，等）. Nanotechnology and Precision Engineering（纳米技术与精密工程）, 2017, 15(6): 438.
[2] Martǎu Gheorghe Adrian, Cǎlinoiu Lavinia-Florina, Vodnar Dan Cristian. Trends in Food Science & Technology，2021, 109：579.
[3] Bai Yuchen, Jian Jinghai, Liu Dehua, et al. Journal of Cleaner Production，2021, 316：128315.
[4] Ohashi M, Omae H, Hashida M, et al. Journal of Chromatography A, 2007, 1138(1-2): 262.
[5] De Jager L S, Perfetti G A, Diachenko G W, et al. Food Chemistry，2008, 107(4): 1701.
[6] XU Xing, PENG Fei-jin, SHU Ping, et al（徐 幸，彭飞进，舒 平，等）. Science and Technology of Food Industry（食品工业科技）, 2016, 37(16): 79.
[7] LI Xiao-dong, JIANG Chen（李晓东，蒋 琛）. Journal of Dairy Science and Technology（乳业科学与技术）, 2013，(2): 34.
[8] Balabin R M, Smirnov S V. Talanta, 2011, 85(1): 562.
[9] WANG Ning-ning, SHEN Bing-hui, GUAN Jian-jun, et al（王宁宁，申兵辉，关建军，等）. Spectroscopy and Spectral Analysis（光谱学与光谱学分析）, 2015, 35(8): 2141.
[10] Waliszewski K N, Pardio V T, Ovando S L. Food Chemistry，2007, 101(3): 1059.
[11] Abid Hussain, Hongbin Pu, Dawen Sun. Critical Reviews in Food Science and Nutrition, 2019, 59(5): 758.
[12] Dicken A, Evans J, Rogers K, et al. Scientific Reports, 2016, 6: 29011.
[13] Callity B D. Elements of X-Ray Diffraction. Addison-Wesley Publishing Company, Inc., London, 1978.
[14] WANG Xiang-ting, GAO Li-feng（王香婷，高利峰）. China Dairy Industry（中国乳品工业），2016，(6): 48.
[15] Celik Sefa, Ozkok Funda, Ozel Aysen E, et al. Journal of Molecular Structure, 2021, 1236：130288.
[16] ZHANG Zheng-yong, LI Li-ping, YUE Tong-tong, et al（张正勇，李丽萍，岳彤彤，等）. Grain Science and Technology and Economy（粮食科技与经济），2018, 43(6)：57.
[17] CHEN Da, HUANG Zhi-xuan, HAN Xi, et al（陈 达，黄志轩，韩 汐，等）. Nanotechnology and Precision Engineering（纳米技术与精密工程），2017, 15(1): 26.
[18] Chu G, Sui Q. Acta Metallurgica Silica，1994，7(3): 179.
