Research on the Influence of Modulation Depth of Phase Sensitive
Detection on Stimulated Raman Signal Intensity and
Signal-to-Noise Ratio
PAN Ke-yu1, 2, ZHU Ming-yao1, 2, WANG Yi-meng1, 2, XU Yang1, CHI Ming-bo1, 2*, WU Yi-hui1, 2*
1. Changchun Institute of Optics, Precision Machinery and Physics, Chinese Academy of Sciences, Changchun 130033, China
2. University of Chinese Academy of Sciences, Beijing 100049, China
Abstract:Stimulated Raman scattering is one of coherent Raman scattering. The signal generated by stimulated Raman scattering is significantly enhanced under the third-order nonlinear effect, and there is no interference from non-resonant background. Its spectrum is almost consistent with the spontaneous Raman spectrum. Therefore, the micro-imaging technology based on stimulated Raman scattering has the advantages of no labeling, high specificity and being non-invasive. It has been successfully used in biological cell imaging and has made many great achievements. Stimulated Raman signal has the same wavelength as excitation luminescence and is easily disturbed by excitation luminescence background noise. In order to solve this problem, the combination of optical modulation and phase-sensitive detection is often used to detect it. In the detection process, modulation depth influences the intensity and signal-to-noise ratio of the stimulated Raman signal. Because of this, this paper deeply analyzes the influence of modulation depth on stimulated Raman signal intensity and signal-to-noise ratio based on relevant theories. At the same time, considering the limitation of cell photodamage threshold on the sum of two excitation optical powers in applications such as bio-spectral imaging, the excitation optical power configuration method to obtain the maximum signal intensity and the best signal-to-noise ratio at different modulation depths is analyzed. By establishing a stimulated Raman experimental system, dimethyl sulfoxide is taken as the research object for experimental verification. The results show that when the stimulated Raman loss is detected under the limitation of photodamage threshold, at the same modulation depth, the signal intensity reaches the strongest when the optical power ratio of pump light to the one of stokes light is 1∶1,and the signal-to-noise ratio reaches the best when the ratio is 1∶2. When the optical power ratio of pump light to the one of stokes light is the same, the intensity and signal-to-noise ratio of stimulated Raman signal decrease with the decrease of modulation depth, and the correlation is approximately linear. The stimulated Raman spectrum of dimethyl sulfoxide obtained from the experiment also verified that the higher the modulation depth, the stronger the spectral signal and the better the signal-to-noise ratio and the better the spectral quality of the whole sample. The research results are the improvement of stimulated Raman microscopy in signal modulation and detection and can provide reference guidance for stimulated Raman spectroscopy detection and cell imaging experiments.
Key words:Phase sensitive detection; Stimulated Raman scattering; Modulation depth; Signal Intensity; Signal-to-Noise ratio; Optimum optical power ratio
潘科宇,朱明尧,王艺蒙,徐 阳,迟明波,吴一辉. 相敏检测调制深度对受激拉曼信号强度及信噪比影响研究[J]. 光谱学与光谱分析, 2023, 43(04): 1068-1074.
PAN Ke-yu, ZHU Ming-yao, WANG Yi-meng, XU Yang, CHI Ming-bo, WU Yi-hui. Research on the Influence of Modulation Depth of Phase Sensitive
Detection on Stimulated Raman Signal Intensity and
Signal-to-Noise Ratio. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1068-1074.
[1] ZHU Ting, LIU Yang, WU Jun, et al(朱 婷,刘 洋,吴 军, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2019, 39(4): 997.
[2] GUO Xiao-yuan, YASHENG Paierhati, LIU Chen-yang, et al(郭晓湲,排尔哈提·亚生,刘晨阳,等). Journal of Fuzhou University(Natural Science Edition)[福州大学学报(自然科学版)], 2021, 49(1): 135.
[3] MIN Wei, YANG Chi, WANG Ping(闵 玮,杨 驰,王 平). Optics & Optoelectronic Technology(光学与光电技术), 2020,(4): 1.
[4] Freudiger C W, Min W, Saar B G, et al. Science, 2008, 322(5909): 1857.
[5] LI Zi-lin, LI Shao-wei, ZHANG Si-lu, et al(李姿霖, 李少伟, 张思鹭,等). Chinese Journal of Lasers(中国激光), 2020, 47(2): 0207005.
[6] GAO Jin-zhan(高晋占). Detection of Weak Signals(微弱信号检测). 3rd Ed.(第3版). Beijing: Tsinghua University Press(北京:清华大学出版社), 2019. 47, 190.
[7] Cheng Jixin, Xie Xiaoliang Sunney. Coherent Raman Scattering Microscopy. Boca Raton: CRC Press, 2013: 7, 219.
[8] Audier X, Heuke S, Volz P, et al. Apl. Photonics., 2020, 5(1): 011101.
[9] Moester M, Ariese F, De Boer J F. J. Eur. Opt. Soc-Rapid., 2015, 10: 15022.
[10] Fu Y, Wang H, Shi R, et al. Opt. Express, 2006, 14(9): 3942.
[11] CHEN Tao, YU Zhi-long, ZHANG Xian-nian, et al(陈 涛, 虞之龙, 张先念, 等). Scientia Sinica:Chimica(中国科学:化学), 2012, 42(1): 1.
[12] YUAN Ya-xiang(袁亚湘). Numerical Methods For Nonlinear Programming(非线性规划数值方法). Shanghai: Shanghai Scientific and Technical Publishers(上海:上海科学技术出版社), 1993.
[13] YU Kuan-xin, DING Xiao-hong, PANG Zhao-guang(俞宽新, 丁晓红, 庞兆广). Acoustooptic Principle and Acoustooptic Devices(声光原理与声光器件). Beijing: Science Press(北京:科学出版社), 2011.
[14] Alan V Oppenheim, Alan S Willsky, S Hamid Nawab. Signal and Systems(信号与系统). 2nd Ed(第2版). Translated by LIU Shu-tang(刘树棠,译). Beijing: Publishing House of Electronics Industry(北京:电子工业出版社),2013.
[15] FEI Ye-tai(费业泰). Theory of Error and Data Processing(误差理论与数据处理). 7th Ed(第7版). Beijing: China Machine Press(北京:机械工业出版社), 2015.