1. State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China
2. College of Food Science and Engineering, Jilin University, Changchun 130062, China
3. College of Plant Protection Key Laboratory of Soybean Disease and Pest Control (Ministry of Agriculture and Rural Affairs)Jilin Agricultural University, Changchun 130118, China
Abstract：Conventional pesticide residue detection still suffers from numerous steps, is long time-consuming, and insufficient delicacy. According to ninhydrin colouring and the principle of localized surface plasmon resonance (LSPR) enhancing absorption, glyphosate in water samples can be detected by ultraviolet-visible spectroscopy (UV-Vis). Furthermore, density functional theory is used to analyze the enhancement mechanism of absorption of purple color dye (PD) products. The PD product displays a maximum absorption of around 570 nm when glyphosate reacts with ninhydrin is detected by UV-Vis. There are slight shifts from 570 nm in the UV-Vis spectrum to 568 nm with a stronger peak when the PD product is absorbed on Ag NPs, and the limit of detection at 2.017 4×10-11 mol·L-1, which is much lower than 6.5×10-7 mol·L-1 limit of detection reported. Gaussian 09 software carried out that the PD product would attach to Ag NPs via an Ag—O bond through ninhydrin’s C═O group vertically. MEP mapping provides C═O group interaction with Ag NPs stable, C═O group coupled C—N comprise a large π-conjugated system in their plane. The color of the C—N group is blue, which suggests that C—N coupled with the C═O group are the chromophore in the PD product. Thus, an indirect detection method derived from ninhydrin can be used for glyphosate detection in water samples. The LSPR effect of Ag NPs enhances the absorption intensity with higher sensitivity than the conventional method.
Key words：Localized surface plasmon resonance; Purple color dye product; Ultraviolet and visible spectrophotometry; Density functional theory; Glyphosate; Ninhydrin
基金资助: National Natural Science Foundation of the People’s Republic of China (32201935, 21773080, 22011540378), Education Department of Jilin Province (JJKH20211127KJ), the Development Program of the Science and Technology of Jilin Province (20190701003GH), the Interdisciplinary Integration and Innovation Project of JLU (JLUX4JC2021Q211)
通讯作者:
赵 冰
E-mail: zhaobing@jlu.edu.cn
作者简介: XU Meng-lei，female, (1988—), a lecturer of State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University e-mail: xumenglei@jlu.edu.cn
引用本文:
徐梦蕾，高 宇，朱 琳，韩晓霞，赵 冰. 基于银纳米粒子的局域表面等离子体共振效应提高草甘膦基于茚三酮间接检测的灵敏度[J]. 光谱学与光谱分析, 2023, 43(01): 320-323.
XU Meng-lei, GAO Yu, ZHU Lin, HAN Xiao-xia, ZHAO Bing. Improved Sensitivity of Localized Surface Plasmon Resonance Using Silver Nanoparticles for Indirect Glyphosate Detection Based on Ninhydrin Reaction. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(01): 320-323.
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