Abstract：Organic fertilizer is an effective means to improve soil physical structure, enhance soil fertility and regulate nutrient balance. However, the effects of organic fertilizer application on the evolution of organic matter and nitrogen in farmland are still unclear. This paper studied the changes of TOC, DOC and inorganic nitrogen contents in soil after applying organic fertilizer. The changes of DOM spectral characteristics of soil after applying organic fertilizer were analyzed using three-dimensional fluorescence spectroscopy. PARAFAC was used to analyze the changes in the relative contents of the fluorescent components in soil DOM in different periods after applying organic fertilizer, and 2D-COS was used to analyze the change sequence of the fluorescent components with time. In addition, the response relationship between the relative content of DOM components and soil nitrogen was studied using the typical correlation analysis method to explore the effects of organic fertilizer application on the evolution of soil organic matter and nitrogen. The results showed that: ① The application of organic fertilizer increased the content of total organic carbon, water-soluble organic carbon and nitrate nitrogen but decreased the content of ammonium nitrogen. ②Three-dimensional fluorescence spectra of soil DOM showed a peak (UV humic acid), M peak (UVA humic acid) and T peak (tryptophan). PARAFAC analysis showed that soil DOM was mainly composed of terrestrial humic acid (C1), typical humic acid (C2) and tryptophan (C3). The results showed that applying organic fertilizer could increase the relative contents of soil C1, C2 and C3 components. The relative contents of C1, C2 and C3 in the soil treated with organic fertilizer increased initially and then decreased, reaching the maximum on the 30th day. The change order of different fluorescence components with time was C1(C2)↑→C3, and humic acid-like changed greatly, and the promotion of humic acid-like by organic fertilizer was significant.③The application of organic fertilizer can improve the bioavailability of soil and reduce the degree of soil humification. BIX value increased first and then decreased after applying organic fertilizer and reached the maximum on the 30th day; The HIX value decreased at first and then went up and reached the minimum on the 30th day. BIX and HIX were negatively correlated (R2=0.732). ④The relative contents of C1, C2 and C3 were positively correlated with nitrate nitrogen and negatively correlated with ammonium nitrogen, and the relative contents of C1 and C2 had a great influence on the contents of nitrate nitrogen and ammonium nitrogen. In conclusion, reasonable application of organic fertilizer can control the transformation of soil organic matter and nitrogen reducing the non-point source pollution of chemical fertilizer.
潘红卫，童文彬，雷宏军，杨 光，史利利. 施加有机肥对农田有机质和氮素演化影响的光谱学分析[J]. 光谱学与光谱分析, 2022, 42(10): 3116-3123.
PAN Hong-wei, TONG Wen-bin, LEI Hong-jun, YANG Guang, SHI Li-li. Spectral Analysis of the Effect of Organic Fertilizer Application on the
Evolution of Organic Matter and Nitrogen in Farmaland. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(10): 3116-3123.
[1] QIN Ji-hong，WANG Shu，LIU Chen, et al(秦纪洪，王 姝，刘 琛, 等). China Environmental Science(中国环境科学), 2019, 39(10): 4321.
[2] HAO Rong, XU Zhao-yu, SHEN Ci-fu, et al(郝 蓉，徐召玉，沈祠福, 等). Ecology and Environmental Sciences(生态环境学报), 2019, 28(6): 1127.
[3] ZHANG Qiang, XI Bei-dou, YANG Jin-jin, et al(张 强，席北斗，杨津津, 等). China Environmental Science(中国环境科学), 2021, 41(2): 763.
[4] MIAO Chuang-he, LÜ Yi-zhong, YU Yue, et al(缪闯和，吕贻忠，于 越, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2020, 40(12): 3832.
[5] HUANG Ting-lin, FANG Kai-kai, ZHANG Chun-hua, et al(黄廷林，方开凯，张春华, 等). Environmental Science(环境科学), 2016, 37(9): 3394.
[6] CUI Bing, GAO Hong-jie, ZHENG Zhao-pei, et al(崔 兵，高红杰，郑昭佩, 等). Journal of Ecology and Rural Environment(生态与农村环境学报), 2021, 37(3): 369.
[7] MIAO Chuang-he，LÜ Yi-zhong(缪闯和，吕贻忠). Soils(土壤), 2021, 53(1): 168.
[8] SHENG Hao, SONG Di-si, WANG Cui-hong, et al(盛 浩，宋迪思，王翠红, 等). Soils(土壤), 2015, 47(6): 1049.
[9] SONG Ge, SUN Bo, JIAO Jian-ying(宋 歌，孙 波，教剑英). Acta Pedologica Sinica(土壤学报), 2007, (2): 288.
[10] ZHOU Guang-wei, ZHANG Wen, MIN Wei, et al(周广威，张 文，闵 伟, 等). Journal of Plant Nutrition and Fertilizers(植物营养与肥料学报), 2015, 21(2): 413.
[11] YANG Zhen-qi, QIN Fu-cang, LI Long, et al(杨振奇，秦富仓，李 龙, 等). Transactions of the Chinese Society of Agricultural Engineering(农业工程学报), 2019, 35(17): 154.
[12] Zepp R G, Sheldon W M, Moran M A. Marine Chemistry, 2004, 89(1): 15.
[13] He W, Hur J. Water Research, 2015, 83: 217.
[14] He W, Jung H, Lee J, et al. The Science of the Total Environment, 2016, 547: 1.
[15] HE Wei，BAI Ze-lin，LI Yi-long, et al(何 伟，白泽琳，李一龙, 等). Acta Scientiae Circumstantiae(环境科学学报), 2016, 36(2): 359.
[16] Stedmon C A，Markager S，Bro R. Marine Chemistry，2003, 82(3-4): 239.
[17] Lapierre J F，Frenette J. Aquatic Science，2009, 71(1): 15.
