Surface-Enhanced Raman Spectra of Oxidation Damnification of Fetal Bovine Serum by Ozone
ZOU Zu-quan1,3,LIU Yan-nan2，3,WU Ying5,LI Qi-nan2,3,CHEN Mei-zong1,3,SUN Hai-ying1,3,Lee Imshik1,3,4
1. Department of Biophysics, School of Physics, Nankai University, Tianjin 300071, China 2. Photonics Center of School of Physics, Nankai University, Tianjin 300071, China 3. The Key Laboratory of Advanced Technique and Fabrication for Weak-Light Nonlinear Photonics Materials, Ministry of Education, Nankai University, Tianjin 300457, China 4. The Key Laboratory of Bioactive Material, Ministry of Education, Nankai University, Tianjin 300071, China 5. China National Academy of Nanotechnology and Engineering, Tianjin 300457, China
Abstract：Fetal bovine serum was treated by ozone for 1 hour and 3 hours before getting its surface-enhanced Raman spectra from 200 to 1 800 cm-1. Treated with ozone for 1 hour, it shows a significant decrease in band intensity. Treated with ozone for 3 hours, the band intensity has a further decrease but not so obviously, which means that oxidation of ozone is short lived. Treated with ozone, the orderly conformations of main chains in protein such as α-helix, β-sheet and β-corner are damaged seriously. Aromatic side chains and C─S of Cys and Met also are damnified greatly. All this means that strong oxidation of ozone results in denaturation, conformational changes and even degradation in protein.
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