1. 贵州医科大学公共卫生学院教育部环境污染监测与控制重点实验室，贵州 贵阳 550004
2. 贵州医科大学临床医学院，贵州 贵阳 550004
3. 贵州医科大学基础医学院，贵州 贵阳 550004
4. 广东工业大学材料与能源学院，广东省功能性软冷凝物质重点实验室，广东 广州 510006
5. Department of Chemistry & Biochemistry, University of Hull, Cottingham Road, Hull, Yorkshire HU6 7RX, UK
A Highly Selective Colorimetric Naked-Eye Probe for Hypochlorite Detection in Water
YU Qing1, CHEN Xiao-li2, ZHANG Qi-long1,3*, LIU Hua3, YANG Xian-jiong3, XU Hong3, HUANG Ya-li3*, FENG Xing4*, REDSHAW Carl5
1. School of Public Health, the Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550004, China
2. School of Clinical Medicine, Guizhou Medical University, Guiyang 550004, China
3. School of Basic Medical Science, Guizhou Medical University, Guiyang 550004, China
4. Guangdong Provincial Key Laboratory of Functional Soft Condensed Matter, School of Material and Energy, Guangdong University of Technology, Guangzhou 510006, China
5. Department of Chemistry & Biochemistry, University of Hull, Cottingham Road, Hull, Yorkshire HU6 7RX, UK
Abstract：The real-time detection and monitoring of hypochlorites (ClO-) in water is highly challenging. An excellent colorimetric “naked-eye” probe photoacid (PAH) was synthesized. PAH was comfirmed using High-Resolution Mass Spectrometry (HRMS), 1H NMR and 13C NMR. The interaction between PAH and ClO- was investigated via UV-Vis absorption spectrophotometry under different pH conditions. The results indicated that PAH was completely soluble in water, PAH displayed a yellow solution in a phosphate buffer with a pH of 2.0 to 5.0, and the maximum absorption peak was at 424 nm. PAH displayed a purple solution in a phosphate buffer with a pH of 6.0~12.0, and the maximum absorption peak was at 532 nm. After adding ClO- to different pH systems, PAH discoloration and the UV-Vis absorption peak disappeared. The probe PAH exhibited specific selectivity and sensitivity for ClO- detection with a low detection limit in the pH 5.0 phosphate buffer. After PAH reacted with ClO-, the absorption peak of the probe at 424 nm gradually decreased, and a new absorption peak appeared at 532 nm. The probe displayed a vivid color-tunable process from yellow to purple then to colorless with a fast response time for ClO- detection. However, other common 33 substances such as metal ions(Li+, Co2+, Cr3+, K+, Cd2+, Pb2+, Ca2+, Hg2+, Ba2+, Cu2+, Mg2+, Ni2+, Zn2+, Al3+ and Fe3+), anions (NO-2, I-, AcO-, ClO-4, SO2-4, CN-, Br-, CO2-3 and F-), reactive oxygen species (ROO·, ·OH, H2O2, ·O-2, tBuOOH, tBuO· and 1O2) and reactive nitrogen species (ONOO- and NO·), did not cause changes in the color of the probe solution and the UV-Vis absorption spectrum. The above species had only a limited effect on detecting the ClO- anion. When they coexisted with ClO-, the probe also showed a similar solution color change, and the absorption peak at 424 nm disappeared. Meanwhile, the probe PAH could quantitatively detect the content of ClO- with a detection limit of 5.39 μmol·L-1 (y=1.586 78-0.524 51x, R2=0.998 52). Furthermore, ClO- concentration in the water system (84 disinfectant and tap water) was analyzed. The average concentration of ClO- ion in the tap water measured by three parallel tests was 7.96 μmol·L-1 with high recoveries rate. It showed that PAH could also be utilized to detect ClO- quantitatively in real water systems.
Key words：Colorimetric probe; Hypochlorite anion; Rapid response; High selectivity
基金资助: the National Natural Science Foundation of China (22065009, 22066007), the Science and Technology Foundation of Guizhou Province ([2019]2792], [2018]5779-14)
作者简介: YU Qing, (1992—), postgraduate in School of Public Health, the Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University e-mail:
1432507000@qq.com
CHEN Xiao-li, (1996—), graduate in School of Clinical Medicine, Guizhou Medical University e-mail:
2422691291@qq.com
引用本文:
余 青，陈晓丽，张奇龙，刘 华，杨先炯，徐 红，黄亚励，冯 星，REDSHAW Carl. 一种用于水中次氯酸根检测的高选择性裸眼比色探针[J]. 光谱学与光谱分析, 2022, 42(01): 322-332.
YU Qing, CHEN Xiao-li, ZHANG Qi-long, LIU Hua, YANG Xian-jiong, XU Hong, HUANG Ya-li, FENG Xing, REDSHAW Carl. A Highly Selective Colorimetric Naked-Eye Probe for Hypochlorite Detection in Water. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(01): 322-332.
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