Fluorescence Spectra Characteristics of Dissolved Organic Matter in Mesophilic Anaerobic Digestion of Pig and Dairy Manure Slurries
LOU Meng-han1, 2, JIN Hong-mei2, 3, 4*, LIANG Dong2, 3, ZHU Yan-yun2, 3, ZHU Ning2, 3, 4, LI Dan-yang2, 3
1. College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China
2. Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
3. Key Laboratory of Crop and Livestock Integrated Farming, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China
4. Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing 210095, China
Abstract:The potential environmental effects of fecal water are determined by the composition changes of substances. Dissolved organic matter (DOM) is an important component of fecal water. In this study, the content of DOM in pig and dairy manure slurries was analyzed by batch mesophilic anaerobic fermentation with a total solid concentration (TS) of 4% and 8%, respectively. DOM’s fluorescence spectrum characteristics and component variation characteristics in raw and digested slurries were analyzed by three-dimensional fluorescence spectroscopy (3DEEM) and parallel factor analysis (PARAFAC). After mesophilic anaerobic fermentation, there sults showed that DOM content in slurries decreased significantly (p<0.001). DOM of manure slurries mainly contained four fluorescent components: tyrosine like, fulvic acid like, tryptophan like and humic acid like. The relative content of humic acid increased significantly (p<0.05), but the relative content of fulvic acid increased only in the treatment of TS=8% but decreased in TS=4%. The humification index of DOM in digested slurries increased significantly (p<0.01), but the humification degree of DOM in pig manure slurries was significantly higher than that in dairy manure slurries. The results can provide theoretical support for evaluating potential environmental effects of livestock manure slurries utilization in farmland.
[1] TAO Xiu-ping, DONG Hong-min(陶秀萍,董红敏). China Agricultural Science and Technology Review(中国农业科技导报), 2017, 19(1): 37.
[2] ZHU Zhi-ping, DONG Hong-min, WEI Sha, et al(朱志平, 董红敏, 魏 莎, 等). Journal of Agricultural Environmental Sciences(农业环境科学学报), 2020, 39(4): 743.
[3] JIN Hong-mei, CHANG Zhi-zhou, YE Xiao-mei, et al(靳红梅, 常志州, 叶小梅, 等). Transactions of the Chinese Society of Agricultural Engineering(农业工程学报), 2011, 27(1): 291.
[4] CHEN Xu, HAN Xiao-zeng, YAN Jun, et al(陈 旭, 韩晓增, 严 君, 等). Soil Bulletin(土壤通报), 2019, 50(03): 732.
[5] Wei Z M, Wang X Q, Zhao X Y, et al. International Biodeterioration & Biodegradation, 2016, 113: 187.
[6] Qin X Q, Yao B, Jin L, et al. Aquatic Geochemistry, 2020, 26(1): 71.
[7] Yan L L, Liu Q P, Liu C, et al. Ecotoxicology and Environmental Safety, 2019, 184:109616.
[8] SHAO Yi-qi, WANG Dian-zhan, YAN Cheng, et al(邵一奇, 王电站, 颜 成, 等). Journal of Nanjing Agricultural University(南京农业大学学报), 2019, 42(4): 713.
[9] LI You-kang, LI Huan, LI Chen-chen(李有康, 李 欢, 李忱忱). Environmental Engineering(环境工程), 2013, 31(S1): 22.
[10] Guo X J, He X S, Zhang H, et al. Microchemical Journal, 2012, 102: 115.
[11] XU Cai-yun, JIN Hong-mei, CHANG Zhi-zhou, et al(许彩云, 靳红梅, 常志州, 等). Acta Agricultural Environmental Sciences(农业环境科学学报), 2016, 35(6): 1167.
[12] Wang K, Li X K, He C, et al. Bioresource Technology, 2014, 168: 222.
[13] LI Yun, WEI Hong-jie, WANG Kan, et al(李 昀, 魏鸿杰, 王 侃, 等). Environmental Science(环境科学), 2019, 40(4): 1751.
[14] Liu J, Chen X F, Li D R N, et al. Journal of Soils and Sediments, 2020, 20(7): 2761.
[15] HAO Xiao-di, ZHOU Peng, CAO Ya-li(郝晓地, 周 鹏, 曹亚莉). Journal of Environmental Engineering(环境工程学报), 2017, 11(1): 1.