Study on Rapid Detection Method of Danofloxacin Mesylate and Ofloxacin Residues in a Chicken Based on Synchronous Fluorescence Spectrum
CHEN Jian1, HUANG Jun-shi1, 2, LIU Mu-hua1, 2, YUAN Hai-chao1, 2, HUANG Shuang-gen1, 2, ZHAO Jin-hui1, 2*, XU Ning1, WANG Ting1, HU Wei1
1. Jiangxi Provincial Key Laboratory of Modern Agricultural Equipment, Jiangxi Agricultural University, Nanchang 330045, China
2. Jiangxi Provincial Collaborative Innovation Center of Key Technologies and Quality and Safety in Post-Harvest Processing of Fruits and Vegetables, Jiangxi Agricultural University, Nanchang 330045, China
Abstract：The rapid detection for danofloxacin mesylate (DFM) and ofloxacin (OFL) residues in chicken were achieved through synchronous fluorescence technology coupled with chemometric methods. First of all, the synchronous fluorescence spectra of DFM standard solution, OFL standard solution, chicken extract without antibiotics and chicken extract containing DFM and OFL were analyzed, and the wavelength difference (Δλ) of DFM and OFL were respectively determined as 130 and 200 nm, and the fluorescence excitation peaks of DFM and OFL were respectively determined as 288 and 325 nm for the detection of DFM and OFL in chicken, respectively. Subsequently, the effects of the concentrations of sodium hydroxide solution and the type of surfactant on the fluorescence intensities were investigated through the single factor test. The best detection conditions of DFM and OFL residues in chicken were as follows: the concentration of sodium hydroxide solution of 0.1 mol·L-1, and the concentration of SDS solution of 0.1 mol·L-1. Finally, the prediction models of DFM and OFL residues in chicken were established using linear regression, partial least squares regression (PLSR), and multiple linear regression (MLR), respectively. The experimental results showed that the comprehensive evaluation of DFM residues’ prediction model of based on the PLSR algorithm was best among these algorithms. The coefficient of determination for the prediction set (R2P) and the root mean square error for the prediction set (RMSEP) were 0.978 3 and 1.934 2 mg·kg-1. The ratio of prediction to deviation (RPD) was 5.876 5. The comprehensive evaluation of the prediction model of OFL residues based on the MLR algorithm was best among these algorithms. The R2P, RMSEP, and RPD were 0.895 0, 3.859 8 mg·kg-1, and 2.509 1, respectively. The adopted method was simple and fast, and could to realize the rapid detection of DFM and OFL residues in chicken.
陈 健，黄俊仕，刘木华，袁海超，黄双根，赵进辉，徐 宁，王 婷，胡 围. 基于同步荧光光谱的鸡肉中甲磺酸达氟沙星和氧氟沙星残留快速检测方法研究[J]. 光谱学与光谱分析, 2021, 41(05): 1367-1372.
CHEN Jian, HUANG Jun-shi, LIU Mu-hua, YUAN Hai-chao, HUANG Shuang-gen, ZHAO Jin-hui, XU Ning, WANG Ting, HU Wei. Study on Rapid Detection Method of Danofloxacin Mesylate and Ofloxacin Residues in a Chicken Based on Synchronous Fluorescence Spectrum. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(05): 1367-1372.
[1] LI Jing-yan, GUO Chun-feng, CUI Li-hui, et al(李婧妍，郭春锋，崔立辉，等). Food Research and Development(食品研究与开发), 2016, 37(22): 153.
[2] YE Sheng-qiang, YANG Yu, WANG Li-xia, et al(叶胜强，杨 宇，王丽霞，等). China Poultry(中国家禽), 2017, 39(16): 69.
[3] WANG Rui, XU Jun, ZHANG Rui-liang, et al(王 瑞，徐 军，张瑞良，等). Journal of Analytical Science(分析科学学报), 2019, 35(2): 152.
[4] GB 31650—2019. National Food Safety Standard—Maximum Residue Limits for Veterinary Drugs in Foods(食品安全国家标准—食品中兽药最大残留限量). National Standards of the People’s Republic of China(中华人民共和国国家标准).
[5] Ministry of Agriculture and Rural Affairs of the People’s Republic of China(中华人民共和国农业农村部). Gazette of the Ministry of Agriculture of the People’s Republic of China(中华人民共和国农业部公报), 2015, (9): 61.
[6] Moghadam N R, Arefhosseini S R, Javadi A, et al. Iran J. Pharm. Res., 2018, 17(4): 1182.
[7] XU Wei-li, NIE Wen, ZHANG Kai-li, et al(徐尉力，聂 稳，张凯丽，等). Food Science(食品科学), 2017, 38(16): 210.
[8] Martins M, Barreto F, Hoff R, et al. Food Addit. Contam Part A, 2015, 32: 333.
[9] HUANG Kun, WU Wan-qin, LUO Tong, et al(黄 坤，吴婉琴，罗 彤，等). Meat Research(肉类研究), 2019, 33(3): 40.
[10] DAI Ting-ting, LIN Hua-ping, DUAN Jie, et al(代婷婷，林华萍，段 婕，等). Chinese J. Anal. Chem.(分析化学), 2016, 44(5): 747.
[11] ZHUANG Yu, MA Chao-qun, WANG Xiao-han, et al(庄 宇，马超群，王小晗，等). Laser Technology(激光技术), 2017, 41(4): 489.
[12] LI Yue-qiu, DOU Hai-yang, WU Jin-tao, et al(李月秋，窦海洋，吴锦涛，等). Food Research and Development(食品研究与开发), 2017, 38(10): 113.
[13] CAI Qi-hong, XU Duan-fang, RUAN Li-qin, et al(蔡其洪，许锻芳，阮丽琴，等). Modern Chemical Industry(现代化工), 2012, 32(10): 98.
[14] GB/T 20366—2006. Method for the Determination of Quinolones in Animal Tissues-LC MS/MS Method(动物源产品中喹诺酮类残留量的测定液相色谱-串联质谱法). National Standards of the People’s Republic of China(中华人民共和国国家标准).
[15] LI Tong-tong(李彤彤). Spectroscopy Studys on the Mechanism of Cefepime with Bovine Serum Albumin and Fluorescence Properties of Sarafloxacin(光谱法研究蛋白与头孢吡肟的作用机理及沙拉沙星荧光性质). Hebei: Hebei University(河北：河北大学), 2017. 7.
[16] XU Jin-gou, WANG Zun-ben(许金钩，王尊本). Fluorescence Analysis(荧光分析法). Beijing: Science Press(北京: 科学出版社), 2006. 44.
[17] LIU Tian-qing, GUO Rong, SHEN Ming, et al(刘天晴，郭 荣，沈 明，等). Acta Physico-chimica Sinica(物理化学学报), 1996，(4): 337.
[18] CHU Xiao-li(褚小立). Molecular Spectroscopy Analytical Technology Combined with Chemometrics and Its Applications(化学计量学方法与分子光谱分析技术). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2011. 56.
[19] ZHAO Xian-de, CHEN Xiao, DONG Da-ming(赵贤德，陈 肖，董大明). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2019, 39(9): 2907.
