Test of the Effect of Different Supercritical Pressure on Andrographolide Crystallization with Chromatography and Spectrum
ZHANG Wen-cheng1,2,ZHANG Xing-yuan2,PAN Jian1
1. Engineering Research Center of Bio-Process of Ministry of Education，Hefei University of Technology,Hefei 230009，China 2. Department of Polymer Science and Engineering,University of Science and Technology of China,Hefei 230026，China
Abstract：With the new technique of supercritical carbon dioxide extraction and crystallization,the experimental materials of 30% and 95% andrographolide were used to investigate the effect of different pressure on crystal distribution on crystallization board,what’s more,the effect of X-ray diffraction and infrared ray spectrum on crystal was tested. The results showed that andrographolide was crystallized on crystallization board in purity gradient with high performance liquid chromatography;the higher the pressure,the higher the crystallization ratio. In addition,the crystal was inclining to directional growth;the chemical structure of andrographolide was not changed with the variety of pressure with infrared ray spectrum analysis.
Key words：Supercritical CO2;Extraction and crystallization;Andrographolide;High performance liquid chromatography;X-ray diffraction;Infrared ray spectrum
张文成1,2,张兴元2,潘见1 . 色谱光谱法测试超临界压力下穿心莲内酯的结晶规律[J]. 光谱学与光谱分析, 2007, 27(08): 1657-1660.
ZHANG Wen-cheng1,2,ZHANG Xing-yuan2,PAN Jian1. Test of the Effect of Different Supercritical Pressure on Andrographolide Crystallization with Chromatography and Spectrum. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(08): 1657-1660.
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