荷移荧光光谱法测定加替沙星

摘要
参考文献
相关文章 (15)

全文: PDF (669 KB)
输出: BibTeX | EndNote (RIS)

摘要：研究了加替沙星(GFLX) 与电子受体四氯对苯醌(TCBQ)之间的荷移反应，建立了测定加替沙星的新方法。实验结果表明，在甲醇-水介质中于45 ℃水浴恒温20 min，GFLX与TCBQ可形成稳定的1∶1电荷转移络合物,其荧光发射强度较GFLX本身有显著的增强。在0.19～5.6 mg·L^-1 浓度范围内,荧光强度与GFLX浓度呈良好的线性关系,检出限为0.005 6 mg·L^-1。用于药物制剂中GFLX的含量测定,其回收率为101.1%～103.9%;标准偏差为1.0%～1.9%。同时建立了测定尿样中GFLX的荷移同步荧光光谱法，GFLX浓度在0.023～3.4 mg·L^-1范围内与荧光强度呈良好的线性关系,检出限为0.003 4 mg·L^-1。用本方法测定尿液中的GFLX，结果与文献值基本一致，回收率为93.9%～101.0%;标准偏差为1.0 %～1.7%。

关键词：加替沙星;四氯对苯醌;荷移反应;荧光光谱

Abstract：A new method for the determination of gatifloxacin (GFLX) has been developed based on the charge transfer reaction between gatifloxacin as the donor and chloranil(TCBQ) as the acceptor by fluorimetry. Experiments show that GFLX has reacted with TCBQ in a mixture of methanol and water at 45 ℃ for 20 min and the complex was formed. The composition ratio was 1∶1. A linear calibration graph was obtained over the GFLX concentration range from 0.19 to 5.6 mg·L^-1 with a detection limit of 0.005 6 mg·L^-1. The method has been applied to the determination of GFLX in the preparation,the recoveries are 101.1%-103.9%,and the relative standard deviation is 1.0%-1.9%. At the same time we proposed a method for the determination of GFLX in urine by charge transfer-synchronous fluorescence spectroscopy. Beer’s law is obeyed in the range of 0.023-3.4 mg·L^-1 of GFLX with a detection limit of 0.003 4 mg·L^-1,the recoveries are 93.9%-101.1%,and the relative standard deviation is 1.0%-1.7%.

Key words：Gatifloxacin;Chloranil;Charge transfer reaction;Fluorescence spectroscopy

收稿日期: 2006-05-10 修订日期: 2006-08-20

中图分类号:

O657.3

通讯作者: 敖登高娃 E-mail: hxgaowa@126.com

引用本文:

敖登高娃,陶玉龙,樊海燕. 荷移荧光光谱法测定加替沙星[J]. 光谱学与光谱分析, 2007, 27(08): 1615-1618.
AODENG Gao-wa,TAO Yu-long,FAN Hai-yan. Fluorescence Spectrometric Determination of Gatifloxacin by Charge Transfer Reaction. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(08): 1615-1618.

链接本文:

https://www.gpxygpfx.com/CN/Y2007/V27/I08/1615

[1] Caroline M Perry,Julia A Barman Balfour,Harriet M Lamb. Drugs,1999,58(4): 683.
[2] DU Yu,GUO Hui-yuan(杜煜,郭惠元). World Notes on Antibiotics(国外医药抗生素分册),2001,22(1): 34.
[3] ZHU Man，WANG Rui，FANG Yi,et al(朱曼,王睿,方毅,等). Chinese Journal of Pharmaceutical Analysis(药物分析杂志)，2003,23(1): 53.
[4] ZHU Hong-da,WANG Zhen-ping,GAO Feng-xi(祝红达，王振平，高逢喜). Chinese Journal of Hospital Pharmacy(中国医院药学杂志)，2006，26(9)： 1180.
[5] BAI Rong,LIU Xia(白荣，刘峡). Journal of Medical Research(医学研究杂志)，2006，35(9)： 71.
[6] XIANG Ping,WU Yu-ling(向萍，吴渝陵). China Pharmaceuticals(中国药业)，2006，15(4)： 17.
[7] LI Hong,ZHANG Li(李红，张锂). China Pharmaceuticals(中国药业)，2006，15(3)： 31.
[8] HUA Jun-yan,TIAN Wei-qiang,ZHU Ya-yan,et al(华俊彦，田伟强，朱雅艳，等). Strait Pharaceuticals Journal(海峡药学)，2006，18(2)： 142.
[9] XU Qin,QIN Xue-lian,DENG Li-dong(徐勤,秦雪莲,邓立东). Journal of Guangdong College of Pharmacy(广东药学院学报),2003,19(3): 234.
[10] YANG Ji-zhang,LIU Rui-qin,YANG Shu-min(杨继章，刘瑞琴，杨树民). Chinese Journal of Hospital Pharmacy(中国医院药学杂志)，2006，26(9)： 1061.
[11] WANG Xiao-ling,LI Dong,NIE Zhong-yue(王晓玲，李东，聂中越). China Pharmacist(中国药师)，2005，8(12)： 1014.
[12] QIN Xue-lian,XU Qin,DENG Li-dong(秦雪莲，徐勤，邓立东). Northwest Pharmaceutical Journal(西北药学杂志)，2004，19(4)： 154.
[13] HUANG Qi-chun(黄其春). Strait Pharmaceutical Journal(海峡药学)，2002，14(2)： 34.
[14] LIAN Ning,ZHAO Hui-chun,SUN Chun-yan(连宁,赵慧春,孙春燕). Chemical Journal of Chinese Universities(高等学校化学学报),2002,23(4): 564.
[15] LIAN Ning,SUN Chun-yan,ZHAO Hui-chun(连宁,孙春燕,赵慧春). Journal of Instrumental Analysis(分析测试学报),2002,21(1): 79.
[16] DAI Gang,AODENG Gao-wa(代钢，敖登高娃). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(7)： 848.
[17] DAI Gang,AODENG Gao-wa(代钢，敖登高娃). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005，25(10)： 1634.