一种用于化学分析的新方法——荧光毛细分析法

摘要
参考文献
相关文章 (15)

全文: PDF (980 KB)
输出: BibTeX | EndNote (RIS)

摘要：提出了一种新方法——荧光毛细分析法(fluorescence capillary analysis，FCA)。FCA法用毛细管做为荧光反应和测定的载体，实现了微量样品的微量分析。本方法特点是操作简便，试样、试剂用量少(<10 μL);毛细管及毛细生物反应器可循环使用，测定成本极低，能大大减少环境污染。该研究基于FCA法测定了核黄素、还原型辅酶Ⅰ，基于固定化酶FCA法测定了酒中乙醇，基于固定化核苷酸探针基因FCA法测定了靶核苷酸，都初步得到了满意的结果。FCA法将可以应用到临床医学、药学、生化、染料工业、发酵食品、环境检测等各个领域中。

关键词：荧光毛细分析法;核黄素;NADH;固定化酶;乙醇;基因

Abstract：A new analytical approach called fluorescence capillary analysis (FCA) has been proposed. FCA method uses a capillary as a carrier for fluorescence reaction and determination,and with this method micro analysis of micro samples has been realized. Its advantages are simple to manipulation,thimbleful of sample and reagent volumes (＜10 μL),repeated use of capillary,and the lowest test cost. FCA method can consumedly decrease the pollution of the environment. Based on FCA and an immobilization enzyme FCA,VB²,NADH,and ethanol in alcohol drinks have been determined. Based on an immobilization probe gene FCA,target nucleotide also has been determined. These results were satisfactory. FCA method can be used for fermentable food,medicine,biochemistry,industry,etc.

Key words：Fluorescence capillary analysis;VB²;NADH;Ethanol;Immobilization enzyme;Gene

收稿日期: 2006-05-10 修订日期: 2006-08-20

中图分类号:

O657.3

通讯作者: 高秀峰 E-mail: lysgxf2005@yahoo.com.cn

引用本文:

李永生¹,高秀峰^2*. 一种用于化学分析的新方法——荧光毛细分析法[J]. 光谱学与光谱分析, 2007, 27(08): 1565-1569.
LI Yong-sheng¹,GAO Xiu-feng^2*. A New Method for Chemical Analysis: Fluorescence Capillary Analysis. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(08): 1565-1569.

链接本文:

https://www.gpxygpfx.com/CN/Y2007/V27/I08/1565

[1] CHEN Ya-hong,CAI Ru-xiu(陈亚红，蔡汝秀). Chinese J. Anal. Chem.(分析化学),2004,32(6): 719.
[2] WEI Rong-hui,HUANG Yan-ping,LI Shan-shan,et al(魏荣慧，黄燕萍，李珊珊，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005，25(11)： 1827.
[3] FANG Guang-rong,SONG Gong-wu,LI Ling,et al(方光荣，宋功武，李玲，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2002，22(4)： 312.
[4] ZHANG Yu-ping,WEI Yong-ju，LI Na,et al(张玉平，魏永巨，李娜，等). Chinese J. Anal. Chem.(分析化学),2004,32(6): 779.
[5] FENG Rui-qin,DING Fen,LIU Yu(冯瑞琴，丁芬，刘昱). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005，25(9)： 1468.
[6] SHEN Jing-shan,SUN Dan-dan,FU Lian-chun,et al(沈景山，孙丹丹，付连春，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005，25(2): 232.
[7] Pascual Reguera I,Guardia Rubio M,Molina Diaz A. Anal. Sci.,2005,21(5): 799.
[8] Tärnhage T,Stromqvist M,Olofsson G,et al. Field Anal. Chem. & Technology,2001,5(4): 171.
[9] Marcela A Segundo,Antonio O S S. Rangel. Anal. Chim. Acta,2002,458: 131.
[10] Matsumoto Y,Kuramitz H,Itoh S,et al. Anal. Sci.,2004,20(3): 219.
[11] KANG Cai-yan,CHEN Yuan-yuan,JIANG Zhi-liang,et al(康彩艳，陈媛媛，蒋治良，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(6)： 1096.
[12] LI Yong-sheng,GAO Xiu-feng(李永生,高秀峰). Chinese Patent(中国专利)，Application No.(申请号)200510021542. 8.
[13] Birkmayer G D,All about NADH,New York： Avery Publishing,2000. 21.
[14] LI Yong-sheng,GAO Xiu-feng(李永生,高秀峰). Chinese Patent(中国专利)，Application No.(申请号)200510021832. 2.
[15] Gulce H,Gulce A,Kavanoz M,et al. Biosensors Bioelectronics,2002,17: 517.
[16] GAO Xiu-feng,LI Yong-sheng,Karube I. Anal. Chim. Acta,2001,443: 257.
[17] GAO Xiu-feng,Ikebukuro K,Karube I,et al. Laboratory Robotics and Automation (Wiley & Sons),1997,9(2): 69.
[18] LI Yong-sheng,GAO Xiu-feng(李永生，高秀峰). J. Sichuan University·Engineering Science Edition(四川大学学报·工程科学版)，2007，39(1)： 74.
[19] LI Yong-sheng,GAO Xiu-feng. Anal. Chim. Acta,2007,588(1): 140.
[20] GAO Xiu-feng,LI Yong-sheng,JIANG Yan-xian(高秀峰，李永生，姜艳霞). Chinese J. Anal. Chem.(分析化学)，2006，34(9)： S220.
[21] Risi J L De,Lyer V R,Brown P O. Science,1997,278: 680.