Effect of Far-Infrared Ceramic Powder on the Interaction Between Essential Oil and BSA
HUANG Fang1, LIU Ming-xue1, 2*, XIONG Jie1, CHEN Lü-qi1, GAO Zhu-xin1, CHEN Hui-ming1, WANG Dan-ni1
1. School of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
2. The Center for Co-Operation and Innovation of Nuclear Waste and Environmental Safety and Provincial Department, Mianyang 621010, China
Abstract：Essential oil and far-infrared ceramic powder(cFIR) are often used in physiotherapy or related health-care products, but the interaction and mechanism between them and biomolecules are less concerned. In this research, the influence and mechanism of cFIR on the interaction between rose essential oil(REO) and bovine serum albumin (BSA) were studied through different spectroscopy methods and infrared thermography under simulated physiological conditions. The results of the fluorescence spectrum show that both the REO and the cFIR can quench the intrinsic fluorescence of BSA. Whether the cFIR exists or not, the quenching mode of the REO to BSA is static quenching. The fitting calculation of Stern-Volmer equation shows that the binding sites n between BSA and REO is increased from 0.55 to 0.96 by adding cFIR, and the binding constant is also significantly increased, which shows that the affinity of REO to BSA can be improved with the existence of cFIR. The effects of cFIR and REO on the secondary structure of BSA were studied through synchronous fluorescence spectrum, three-dimensional fluorescence spectrum and Fourier transform infrared spectrum. The results show that they can cause the increase of hydrophobicity around the protein and have little effect on the conformation of BSA; in addition, cFIR and REO show the synergistic effect on the quenching of BSA intrinsic fluorescence through the formation of association compounds with BSA. Critical distance (R0) and binding distance (r) were calculated based on Förster’s non-radiative energy transfer theory. The binding distance (r) between BSA and REO changes from 1.445 nm to 1.453 nm after the addition of cFIR, R0 is basically unchanged and R0 is less than r. These results suggest that whether the cFIR exists, the static quenching of BSA fluorescence by REO is accompanied by non-radiative energy transfer. The cFIR mainly improves the affinity of REO to BSA by changing the conformation of BSA due to different non-competitive binding sites between the cFIR and REO on BSA molecular surface. Infrared thermal imaging techniques was used to explore the interaction of REO and cFIR with macromolecules. Infrared thermal imaging results show that REO can reduce the temperature of biomolecules, while cFIR can increase the temperature of biomolecules. The addition of the cFIR can increase a response temperature of BSA-REO system, which indicates the thermal effect will increase (p<0.05). The above results show that the cFIR with nano-size effect and far-infrared radiation can affect the microenvironment of the system by forming association compounds when it interacts with REO, thus affect the fluorescence characteristics and energy transfer of biomolecules. The results of this study can provide a reference for further application of essential oil combined with cFIR in physiotherapy and mechanism research.
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